Mouse anti-Human CD166 Monoclonal Antibody | anti-CD166 antibody

CD166, Human, mAb AZN-L50

Cat. Num. AAA584070

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Human
• Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Protein G
• Synonyms: CD166; Monoclonal Antibody; Human; mAb AZN-L50; Activated leukocyte cell adhesion molecule; ALCAM; CD166.; anti-CD166 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Purity/Purification: Protein G
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Concentration: 100 ug/mL (varies by lot)
• Sequence Length: 133

• Applicable Applications for anti-CD166 antibody: F, Flow Cytometry (FC), FS, Immunoflurescence (IF), Immunoprecipitation (IP), P, Western Blot (WB)
• Application Notes: Application Notes: IHC-F:- Tissue sections were fixed in acetone; Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Immunogen: CD166 expressing K562 cells
• Endotoxin: <0.4EU/mg
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Testing Data (TD)

Immunohistochemistry (IHC)

(IHC: Human CD166 (ALCAM) in bone marrow cells. Staining with monoclonal antibody AZN-L50.)

Related Product Information for anti-CD166 antibody

Product Description: The monoclonal antibody AZN-L50 recognizes activated leukocyte cell adhesion molecule (ALCAM), a member of the immunoglobulin superfamily and has a characteristic VVC2C2C2 domain structure. ALCAM is a type I transmembrane protein of 100 kDa with five extracellular immunoglobulin-like domains and a short cytoplasmic tail. ALCAM mediates homotypic ALCAM-ALCAM interactions and heterotypic interactions with the T-cell antigen CD6. Homotypic interactions are involved in the development and maintenance of tissue architecture and tumor progression. Heterotypic interactions initiate and stabilize T-cell-dendritic interactions affecting T-cell activation.
ALCAM is secreted as a NH2-terminal fragment of 30 kD. Soluble ALCAM (sALCAM) induces divergent biological signals and responses.
ALCAM shows a characteristic, temporal and spatial distribution in development of a wide variety of tissues and cells in health and disease. ALCAM is expressed on a wide variety of cells, within the leukocyte population its expression is particularly high on dentritic cells. ALCAM is involved in various physiological processes including hematopoieses, thymus development, the immune response, neurite extension, neural cell migration, embryogenesis, neurogenesis, angiogenesis, osteogenesis and is a marker for pluripotent mesenchymal stem cells.
The monoclonal antibody AZN-L50 completely blocks homophilic ALCAM-ALCAM interaction.

References

1. Kempen van, L et al; Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. J Biol Chem 2001, 276: 25783 2. Lunter, P et al; Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD), a novel actor in invasive growth, controls matrix metalloproteinase activity. Cancer Res 2005, 65: 8801
3. Zimmerman, A et al; Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood 2006, 107: 3212
4. Riet te, J et al; Distinct kinetic and mechanical properties govern ALCAM-mediated interactions as shown by single-molecule force spectroscopy. J Cell Sci 2007, 120: 3965
5. Bladergroen, B et al; Spatially separated distribution and highly flexible expression of adhesion molecules facilitates dynamic hematopoiesis. J Med Sci 2007, 7:1239
6. Kilsdonk van, J et al; Attenuation of melanoma invasion by a secreted variant of activated leukocyte cell adhesion molecule. Cancer Res 2008, 68:3671

NCBI and Uniprot Product Information

• NCBI GI #: 343168776
• NCBI GeneID: 214
• NCBI Accession #: NP_001230212.1
• NCBI GenBank Nucleotide #: NM_001243283.1
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: CD166 antigen isoform 4
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion