Mouse anti-Human VE-Cadherin Monoclonal Antibody | anti-CDH5 antibody

VE-Cadherin, Human, mAb BV9, FITC

Cat. Num. AAA584045

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: VE-Cadherin; Monoclonal Antibody; Human; mAb BV9; FITC; 7B4 antigen; Cadherin-5; CD144; anti-CDH5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide
• Sequence Length: 784

• Applicable Applications for anti-CDH5 antibody: F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF), Immunopreciptation (IP), Western Blot (WB)
• Application Notes: Application Notes: F: Acetone fixed sections were blocked with horse serum and incubated with antibody BV9 for 30 minutes (Ref.2).; Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
• Conjugate: FITC
• Positive Control: HUVECs grown on coverslips
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CDH5 antibody

Product Description: The monoclonal antibody BV9 binds to the extracellular domain (EC3-EC4) of human VE-cadherin (vascular endothelial cadherin). Endothelial cells control the passage of plasma constituents and circulating cells from blood to the underlying tissues. VE-cadherin is of vital importance for the maintenance and control of endothelial cell contacts. Mechanisms that regulate VE-cadherinmediated adhesion are important for the control of vascular permeability and leukocyte extravasation. VE-cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Therefore, VE-cadherin is also essential during embryonic angiogenesis. The specialized function of VE-cadherin is lost or impaired in several pathological conditions - including inflammation, sepsis, ischemia and diabetes - which leads to severe, and sometimes fatal, organ dysfunction. Furthermore, abnormal increase in vascular permeability is often observed in pathological conditions, such as tumor-induced angiogenesis, macular degeneration, allergy, and brain stroke.
Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherent junctions. In vascular endothelium, adherent junctions are mainly composed of VE-cadherin, an adhesive receptor that is able to self-associate at endothelial cellcell contacts. VE-cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. VE-cadherin belongs to the cadherin super-family of cellcell adhesion molecules, which are encoded by more than 200 genes in the human genome. Classical cadherins are Ca2+-dependent, homophilic, cell to cell adhesion molecules expressed in nearly all cells within solid tissues. Cadherins form a core adhesion complex that consists of a cadherin dimer, binding through its extracellular region to another dimer of cadherins expressed in adjacent cells, while its intracellular region is anchored to the plasma membrane and linked to the cytoskeleton. The VE-cadherin extracellular domain consists of five cadherin-type repeats, called EC (extracellular cadherin) domains that are bound together by calcium ions in a rod-like structure.

References

1. Navarro, P et al; Catenin-dependent and -independent functions of vascular endothelial cadherin. J Biol Chem 1995, 270: 30965 2. Martin-Padura, I et al;- Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haomangiomas. J path 1995, 175: 51
3. Breviario, F et al; Functional properties of human vascular endothelial cadherin (7B4/Cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb 1995, 15: 1229-
4. Gill, M et al. Vascular trauma induces rapid but transient mobilization of VEGFR2+AC133+ endothelial precursor cells. Circ Res 2001, 88: 167
5. Spagnuolo, R et al. Gas1 is induced by VE-cadherin and vascular endothelial growth factor and inhibits endothelial cell apoptosis. Blood 2004, 103: 3005
6. Kuchler, A et al. Nuclear il-33 is generally expressed in resting endothelium but rapidly lost upon angiogenic or proinflammatory activation. AM J path. 2008, 173: 1229
7. Abraham, S et al. VE-cadherin mediated cell-cell interaction suppresses sprouting via signalling to MLC2 phosphorylation. 2009, 19: 668
8. Vallon, M et al. Tumor endothelial marker 5 expression in endothelial cells during capillary marphogenesis is induces by the small GTPase Rac and mediates contact inhibition of cell proliferation. Exp Cell res. 2010, 316: 412

NCBI and Uniprot Product Information

• NCBI GI #: 599834
• NCBI GeneID: 1003
• UniProt Accession #: P33151; Q4VAI5; Q4VAI6
• Molecular Weight: 74,499 Da
• NCBI Official Full Name: VE-cadherin
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: tight junction; adherens junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: negative regulation of cell proliferation; intercellular junction assembly and maintenance; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation