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Structure

DPPH, inhibitor

DPPH

Purity
99.25%
Synonyms
DPPH; 2; 2-Diphenyl-1-picrylhydrazyl; inhibitor
Ordering
For Research Use Only!
Purity/Purification
99.25%
Form/Format
Solid. Powder
Solubility
DMSO: 24mg/mL (60.86mM) Need ultrasonic
(<1mg/ml refers to the product slightly soluble or insoluble)
Formula
C18H12N5O6
SMILES
[O-][N+](=O)c1cc(c(NN(c2ccccc2)c2ccccc2)c(c1)[N+]([O-])=O)[N+]([O-])=O
CAS Number
1898-66-4
Target & IC50
Others
In Vitro
DPPH shows a strong absorption band at 517nm. Due to its odd number of electrons, the solution presents a dark purple color, and the absorption disappears as the electron pairs are detached. The resulting decoloration is stoichiometric and depends on the number of electrons absorbed. Alcohol solutions of 0.5mM are dense in color, and at this concentration, the Lambert-Beer law is followed within the effective range of absorption.DPPH is a fast, simple, inexpensive and widely used method for determining the ability of compounds to act as free radical scavengers or hydrogen donors and for evaluating the antioxidant activity of foods.It can also be used to quantify antioxidants in complex biological systems, solid or liquid samples. The method was simple and easy to determine the total antioxidant capacity and free radical scavenging activity of fruit and vegetable juice. The antioxidant properties of wheat grains and bran, vegetables, conjugated linoleic acid, herbal medicine, edible seed oil and flour in ethanol, acetone, methanol, ethanol and benzene were successfully studied.It is a simple method for the determination of the antioxidant properties of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxyaromatic compounds in olive oil, fruits, fruit juices and wines.
Preparation and Storage
Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Structure

Structure
Related Product Information for DPPH, inhibitor
DPPH is a cell-permeable, stable free radical that is commonly used to evaluate the ability of compounds to act as free radical scavengers or hydrogen donors and to measure the antioxidant activity of tissue extracts
References
Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22.
Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22.

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Product Notes

The DPPH (Catalog #AAA5753043) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DPPH, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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