Rabbit SH2B1 Polyclonal Antibody | anti-SH2B1 antibody

SH2B1 (SH2B Adapter Protein 1, PSM, SH2B) (FITC)

Cat. Num. AAA6437411

• Gene Names: SH2B1; PSM; SH2B
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: SH2B1; Polyclonal Antibody; SH2B1 (SH2B Adapter Protein 1; PSM; SH2B) (FITC); anti-SH2B1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes SH2B1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-SH2B1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human SH2B1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SH2B1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using SH2B1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-SH2B1 antibody

Antibodies; Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 224926826
• NCBI GeneID: 25970
• NCBI Accession #: NP_001139267.1
• NCBI GenBank Nucleotide #: NM_001145795.1
• UniProt Accession #: Q9NRF2; Q96FK3; Q96SX3; Q9NRF1; Q9NRF3; Q9P2P7; Q9Y3Y3; A8K2R7
• Molecular Weight: 72,188 Da
• NCBI Official Full Name: SH2B adapter protein 1 isoform 1
• NCBI Official Synonym Full Names: SH2B adaptor protein 1
• NCBI Official Symbol: SH2B1
• NCBI Official Synonym Symbols: PSM; SH2B
• NCBI Protein Information: SH2B adapter protein 1; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2-B signaling protein; pro-rich, PH and SH2 domain-containing signaling mediator
• UniProt Protein Name: SH2B adapter protein 1
• Protein Family: SH2B adapter protein
• UniProt Gene Name: SH2B1
• UniProt Synonym Gene Names: KIAA1299; SH2B; PSM
• UniProt Entry Name: SH2B1_HUMAN

NCBI Description

This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]

Uniprot Description

SH2-B-beta: an adaptor protein that specifically activates JAK2 and serve as adapter proteins for JAK1, -2 and -3. YXXL motifs in SH2-Bbeta are phosphorylated by JAK2, JAK1, and platelet-derived growth factor receptor and are required for membrane ruffling.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: membrane; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding

Biological Process: lamellipodium biogenesis; positive regulation of mitosis; blood coagulation