Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human Mamma tumor Her2Neu +
)
anti-Human Neu-Oncogen (C-erb B2) Monoclonal Antibody
Mouse anti Neu-Oncogen (C-erb B2)
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Neu-Oncogen (C-erb B2); Monoclonal Antibody; Mouse anti Neu-Oncogen (C-erb B2); anti-Neu-Oncogen (C-erb B2) antibody
Host
Mouse (Balb/c)
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G6
Specificity
9G6 Immunoprecipitates a 180 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of Human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-Neu-Oncogen (C-erb B2) antibody
Electron microscopy, Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
9G6 is useful for immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen and paraffin embedded tissues. Optimal antibody dilution should be determined by titRation.
Source Note
9G55 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a synthetic peptide from the C-terminus of Human c-erbB-2 protein.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human Mamma tumor Her2Neu + )
Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human Mamma tumor Her2Neu +
)
Related Product Information for anti-Neu-Oncogen (C-erb B2) antibody
C-erbB-2 (erythroblastosis oncogene B), also known as HER2 (Human Epidermal Growth Factor Receptor 2), or Neu, CD340 and p185 is a protein that in Humans is encoded by the ERBB2 gene. AmplifiCation or overexpression of this gene has been shown to play an important role in the pathogenesis and progression of certain aggressive types of breast cancer, as well as many other epithelial malignancies and brain tumors. In recent years it has become an important biomarker and target of therapy for disease. ERBB2 is a known proto-oncogene loCated at the long arm of Human chromosome 17 (17q21-q22). The oncogene was found to code for EGFR. Gene cloning showed that HER2, Neu and ErbB-2 are all encoded by the same gene. The ErbB family is composed of plasma membrane-bound receptor tyrosine kinases, that contain an extracellular ligand binding domain, a transmembrane domain and an intracellular domain that can interact with a multitude of signaling molecules. HER2 can heterodimerise with any of the other three receptors and is considered to be the preferred dimerisation partner of the other ErbB receptors. Dimerisation results in the autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptors and initiates a variety of signaling pathways.
Product Categories/Family for anti-Neu-Oncogen (C-erb B2) antibody
References
Van de Vijver, M.J. Peterse, J.L. Mooi, W.J. Wisman, P. Lomans, J. Dalesio, O. and Nusse, R. (1988). Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer. N Engl J Med. 319, 1239-45.
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Product Notes
The Neu-Oncogen (C-erb B2) (Catalog #AAA570277) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Neu-Oncogen (C-erb B2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neu-Oncogen (C-erb B2) can be used in a range of immunoassay formats including, but not limited to, Electron microscopy, Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Immunoprecipitation (IP), Western Blot (WB). 9G6 is useful for immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen and paraffin embedded tissues. Optimal antibody dilution should be determined by titRation. Researchers should empirically determine the suitability of the Neu-Oncogen (C-erb B2) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neu-Oncogen (C-erb B2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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