BMI1 Monoclonal Antibody
Mouse anti Human BMI1
Reactivity
Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
BMI1; Monoclonal Antibody; Mouse anti Human BMI1; anti-BMI1 antibody
Host
Mouse (Balb/c)
Reactivity
Human, Mouse, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
229F6
Specificity
229F6 preferentially associates with several distinct heterochromatic domains in tumor cell lines. In both primary and tumor cell lines a marked cell cycleregulation of Pc-G-chromatin interaction is observed.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-BMI1 antibody
Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
229F6 is useful for immunoprecipitation, western blot and immunohistochemistry on frozen sections. Optimal antibody dilution should be determined by titRation.
Source Note
229F6 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Bmi1 protein corresponding to residues 1-202 of Mouse Bmi-1 acterial fusion protein of membrane proximal non repeat domain of Human episialin.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-BMI1 antibody
BMI1 polycomb ring finger oncogene, also known as BMI1, is a protein which in Humans is encoded by the BMI1 gene. BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 is rapidly recruited to sites of DNA damage and it sustains for over than 8h. Loss of BMI1 leads to radiation sensitive and impaired repair of DNA double-strand breaks by homologous recombination. Bmi1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi1 is also thought to inhibit ageing in neurons through the suppression of p53. In primary and tumor cells, nuclear BMI1 shows a fine-grain distribution over chromatin, usually dense in interphase nuclei and significantly weaker along mitotic chromosomes. In addition, BMI1 preferentially associates with several distinct heterochromatic domains in tumor cell lines. Bmi1 seems to play an important role in several types of cancer, such as bladder, skin, prostate, breast, ovarian, colorectal as well as hematological malignancies. Its amplifiCation and overexpression is especially pronounced in mantle cell lymphomas. In both primary and tumor cell lines a marked cell cycle regulation of Pc-G-chromatin interaction is observed: nuclear BMI1-staining dissipates in late S phase and is reestablished early in G1-phase. Chromatin-association of BMI1 inversely correlates with its phosphorylation status in a cell cycle-dependent fashion: at G1/S, hypophosphorylated. BMI1 is specifically retained in the chromatin-associated nuclear protein fraction, whereas during G2/M, phosphorylated BMI1 is not chromatin bound.
Product Categories/Family for anti-BMI1 antibody
References
Jan Willem Voncken, Dieter Schweizer, Louise Aagaard, Lydia Sattle, Michael F. Jantsch and Maarten van Lohuizen. Chromatin-association of the Polycomb group protein BMI1 is cell cycle regulatedand correlates with its phosphorylation status. J. of Cell Science 112: 4627-4639, 1999. Mark J. Alkema, Marieke Bronk, Els Verhoeven, Arie Otte, Laura J. van 't Veer, Anton Berns and Maarten van Lohuizen. IdentifiCation of BMI1-interacting proteins as constituents of a multimeric mammalian Polycomb complex. Genes Dev. 11: 226-240, 1997.
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Product Notes
The BMI1 (Catalog #AAA570231) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human BMI1 reacts with Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP), Western Blot (WB). 229F6 is useful for immunoprecipitation, western blot and immunohistochemistry on frozen sections. Optimal antibody dilution should be determined by titRation. Researchers should empirically determine the suitability of the BMI1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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