Rabbit ITLN1 Polyclonal Antibody | anti-ITLN1 antibody

ITLN1 Antibody

Cat. Num. AAA9603641

• Gene Names: ITLN1; HL1; LFR; HL-1; INTL; ITLN; hIntL; omentin
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ITLN1; Polyclonal Antibody; ITLN1 Antibody; Endothelial lectin HL 1; Endothelial lectin HL-1; FLJ20022; Galactofuranose binding lectin; Galactofuranose-binding lectin; hIntL; HL 1; HL1; Intelectin; Intelectin 1; Intelectin-1; Intestinal lactoferrin receptor; INTL; ITLN; ITLN-1; ITLN1_HUMAN; LFR; Omentin; OTTHUMP00000027882; anti-ITLN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ITLN1 antibody detects endogenous levels of ITLN1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 313

• Applicable Applications for anti-ITLN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human ITLN1.
• Subcellular Location: Cell Membrane. Secreted. Enriched in lipid rafts.
• Tissue Specificity: Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level) (PubMed:16531507). Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of human heart tissue, using ITLN1 antibody.)

Immunohistochemistry (IHC)

(MBS9603641 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-ITLN1 antibody

Function: Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:11313366, PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1, 2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed:26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed:26148048). Does not bind human glycans (PubMed:26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed:16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed:16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed:11747454, PubMed:23921499).
Subunit Structure: Homotrimer; disulfide-linked (PubMed:11313366, PubMed:17621593, PubMed:26148048). May interact with LTF (PubMed:11747454, PubMed:23921499).
Post-translational Modifications: N-glycosylated.

NCBI and Uniprot Product Information

• NCBI GI #: 31542986
• NCBI GeneID: 55600
• NCBI Accession #: NP_060095.2
• NCBI GenBank Nucleotide #: NM_017625.2
• UniProt Accession #: Q8WWA0; Q5IWS4; Q5VYI4; Q6YDJ3; Q9NP67
• Molecular Weight: Observed: 35-40 kDa; Predicted: 35 kDa
• NCBI Official Full Name: intelectin-1
• NCBI Official Synonym Full Names: intelectin 1
• NCBI Official Symbol: ITLN1
• NCBI Official Synonym Symbols: HL1; LFR; HL-1; INTL; ITLN; hIntL; omentin
• NCBI Protein Information: intelectin-1
• UniProt Protein Name: Intelectin-1
• Protein Family: Intelectin
• UniProt Gene Name: ITLN1
• UniProt Synonym Gene Names: INTL; ITLN; LFR; ITLN-1

Uniprot Description

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:11313366, PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed:26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed:26148048). Does not bind human glycans (PubMed:26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed:16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed:16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed:11747454, PubMed:23921499).