MMP-14 active protein

MMP-14 (catalytic domain) (human), (recombinant)

Cat. Num. AAA566600

• Purity: Partially purified by single-step affinity chromatography and gel filtration
• Synonyms: MMP-14; MMP-14 (catalytic domain) (human); (recombinant); MMP-14 active protein

• Host: E Coli
• Purity/Purification: Partially purified by single-step affinity chromatography and gel filtration
• Form/Format: Liquid. In 50mM TRIS, pH7.5, containing 5mM calcium chloride, 300mM sodium chloride, 20uM zinc chloride, 0.5% Brij-35, and 30% glycerol

• Source Note: Active Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase 1, MT1-MMP) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-14 (Tyr112-Arg298, NM_004995) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-14 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected
• Activity: Preincubation of MMP-14 catalytic domain at 13.6nM with the broad-spectrum inhibitor GM6001 at 20nM for 1 hour completely inhibits enzymatic activity
• Specific Activity: >=70U/ug. One U=100 pmol/min at 37 degree C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100uM) as substrate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Product Categories/Family for MMP-14 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: P50281
• Molecular Weight: 225kDa
• UniProt Protein Name: Matrix metalloproteinase-14
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
• UniProt Entry Name: MMP14_HUMAN

Uniprot Description

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.

Protein type: Protease; EC 3.4.24.80; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; melanosome; plasma membrane

Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity

Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; male gonad development; negative regulation of focal adhesion formation; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; branching morphogenesis of a tube; regulation of transcription, DNA-dependent; ovarian follicle development; response to mechanical stimulus; response to estrogen stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; angiogenesis; response to oxidative stress; astrocyte cell migration; lung development; endochondral ossification; positive regulation of cell migration

Disease: Winchester Syndrome