Dkk-1 recombinant protein

Dkk-1 (mouse), (recombinant) (His-tag)

Cat. Num. AAA565898

• Gene Names: Dkk1; mdkk-1
• Applications: Western Blot
• Purity: >=95% (SDS-PAGE; Western blot); Purified by multi-step chromatography
• Synonyms: Dkk-1; Dkk-1 (mouse); (recombinant) (His-tag); Dkk-1 recombinant protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE; Western blot); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS, pH8.5, containing 250mM sodium chloride, 2mM DTT, and 1mM EDTA

• Applicable Applications for Dkk-1 recombinant protein: Western Blot (WB)
• Source Note: Mouse Dkk-1 is fused at the N-terminus to a His-tag
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for Dkk-1 recombinant protein

Dkk-1 (Dickkopf or Dickkopf-1) is a secreted inhibitor of the Wnt/canonical signaling pathway which mediates a variety of cellular processes. Wnt proteins are extracellular signaling molecules that initiate activation of the canonical pathway by binding low-density lipoprotein receptor-related protein 5&6 (LRP5/6) coreceptors, triggering a cascade of events that stabilize beta-catenin. Wnts mediate osteoblast development and cell-to-cell interactions during embryogenesis, and are also responsible for regulating bone mass in adults during normal bone remodeling processes. Wnts are also found to be expressed during the stationary phase of the cell cycle and are thought to control expansion of bone marrow stromal cells preventing overpopulation in marrow. There are at least two families of secreted inhibitors of Wnt signaling: the secreted frizzled-related protein family and the Dickkopf family. The mouse Dkk-1 protein consists of 272 amino acids including a 31 amino acid N-terminal signal peptide. Mature native Dkk-1 has a predicted molecular weight of 26kD and an apparent molecular weight of approximately 38-42kD by SDS-PAGE and western blot analysis. Dkk-1 disrupts the canonical pathway by binding to the LRP5/6 coreceptors, preventing interaction with Wnt and facilitating degradation of beta-catenin, whichuLtimately results in osteolytic breakdown of bone tissue. Wnt signaling dysregulation has been implicated in cancer of the colon and stomach. Gonzalez-Sancho reported that the Wnt/beta-catenin pathway is downregulated by the induction of Dkk-1 expression, a mechanism that is lost in colon cancer, suggesting that Dkk-1 acts as a tumor suppressor gene in this cancer type. Dkk-1 proteins are expressed during log phase of the cell cycle, suggesting that dysregulated Dkk-1 could be responsible for the growth of some malignancies. The Dkk-1 gene has also been determined to be a target of p53, as Cappuccio suggests it is a necessary requirement for the execution of cell death. Tian reports that elevated Dkk-1 levels in bone marrow, plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of Dkk-1, and were associated with the presence of focal bone lesions. A study reported serum levels of Dkk-1 in patients with multiple myeloma before autologous stem cell transplantation. The range of Dkk-1 serum levels in this group of patients was 6.4-234.8 ng/mL with a mean of 63.6 ng/mL and a standard deviation of 77.3. Hall et al. suggest that Dkk-1 may act like a switch on prostate cancer cell activity, causing cells to change from osetoblastic to a highly osteolytic cell line.

Product Categories/Family for Dkk-1 recombinant protein

Cellular Analysis; Cell & Tissue Markers; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 408360053
• NCBI GeneID: 13380
• UniProt Accession #: O54908; Q80UL5
• Molecular Weight: 29,298 Da
• NCBI Official Full Name: Dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf homolog 1 (Xenopus laevis)
• NCBI Official Symbol: Dkk1
• NCBI Official Synonym Symbols: mdkk-1
• NCBI Protein Information: dickkopf-related protein 1; dkk-1; dickkopf-1; dickkopf-like protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• UniProt Gene Name: Dkk1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; mDkk-1
• UniProt Entry Name: DKK1_MOUSE

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: receptor antagonist activity; protein binding; low-density lipoprotein receptor binding

Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; Wnt receptor signaling pathway; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; multicellular organismal development; regulation of receptor internalization; regulation of Wnt receptor signaling pathway; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; hair follicle development; negative regulation of mesodermal cell fate specification; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; endoderm development; embryonic limb morphogenesis