Dkk-1 recombinant protein

Dkk-1, 32-266aa, Human, His tag, Baculovirus

Cat. Num. AAA203274

• Gene Names: DKK1; SK; DKK-1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: Dkk-1; 32-266aa; Human; His tag; Baculovirus; Dickkopf-related protein 1; Dickkopf-1; hDkk-1; SK; Dickkopf-related protein 1 Dickkopf 1; Dickkopf 1 like; Dickkopf homolog 1; Dickkopf related protein 1; DKK 1; hDkk 1; DKK1; Dkk-1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 50% glycerol, 2mM DTT, 2mM EDTA, 0.1mM PMSF
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: ADPTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGSHHH HHH
• Sequence Length: 266

• Applicable Applications for Dkk-1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Dkk-1 recombinant protein

Dickkopf-related protein 1, also known as Dkk-1, is a member of the Dkk protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated level of Dkk-1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Recombinant human Dkk-1 protein, fused to His-tag at C-terminus, was expressed ininsect cell using baculovirus expression system and purified by using conventional chromatography.

Product Categories/Family for Dkk-1 recombinant protein

Signal Transduction

References

Krupnik, V.E. et al. (1999) Gene 238:301-13 ; Nusse, R. et al. (2001) Nature 411:255-6 ; Mao, J. et al. (2001) Mol. Cell 7:801-9;

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374
• NCBI GenBank Nucleotide #: NM_012242.3
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 27.8kDa (253aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• UniProt Gene Name: DKK1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; hDkk-1

NCBI Description

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

Uniprot Description

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity ().