Growth Differenntiation Factor-5 (GDF-5) Recombinant Protein | GDF-5 recombinant protein

Recombinant Human Growth Differenntiation Factor-5 (GDF-5 Human)

Cat. Num. AAA555526

• Gene Names: GDF5; OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
• Reactivity: Human
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis bySDS-PAGE.
• Synonyms: Growth Differenntiation Factor-5 (GDF-5); Recombinant Human Growth Differenntiation Factor-5 (GDF-5 Human); GDF-5 recombinant protein

• Host: E. Coli
• Reactivity: Human
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis bySDS-PAGE.
• Form/Format: The protein was lyophilized without any additives.
• Sequence: APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

• Protein Content: Protein quantitation was carried out by two independent methods: ; 1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). ; 2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
• Reconstitution: It is recommended to reconstitute the lyophilized Growth Differentiation Factor 5 in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for GDF-5 recombinant protein

Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 2748 Dalton. To enable bacterial expression of GDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys. GDF5 is purified by proprietary chromatographic techniques.

Product Categories/Family for GDF-5 recombinant protein

Proteins; Growth/Differentiation Factors and Small Molecules; Axon Growth and Guidance; GDF-5

NCBI and Uniprot Product Information

• NCBI GI #: 611435007
• NCBI GeneID: 8200
• NCBI Accession #: NP_000548.2
• NCBI GenBank Nucleotide #: NM_000557.4
• NCBI Official Full Name: growth/differentiation factor 5 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 5
• NCBI Official Symbol: GDF5
• NCBI Official Synonym Symbols: OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
• NCBI Protein Information: growth/differentiation factor 5
• UniProt Protein Name: Growth/differentiation factor 5
• UniProt Gene Name: GDF5
• UniProt Synonym Gene Names: BMP14; CDMP1; GDF-5; BMP-14; CDMP-1; LAP-4; LPS-associated protein 4
• UniProt Entry Name: GDF5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; forelimb morphogenesis; BMP signaling pathway; hindlimb morphogenesis; extracellular matrix organization and biogenesis; positive regulation of chondrocyte differentiation; cell-cell signaling; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; chondrocyte differentiation; regulation of multicellular organism growth; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; growth; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation

Disease: Chondrodysplasia, Grebe Type; Acromesomelic Dysplasia, Hunter-thompson Type; Brachydactyly, Type A1, C; Brachydactyly, Type A2; Symphalangism, Proximal, 1b; Fibular Hypoplasia And Complex Brachydactyly; Brachydactyly, Type C; Osteoarthritis Susceptibility 5; Multiple Synostoses Syndrome 2