WISP3 active protein

WISP3

Cat. Num. AAA553639

• Gene Names: CCN6; PPD; LIBC; PPAC; WISP3; WISP-3
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: WISP3; WISP3 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L
• Sequence Length: 372

• Applicable Applications for WISP3 active protein: SDS-PAGE
• Application Notes: Recombinant WISP3 migrates as an approximately 36 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human WISP3 mature chain including a C-terminal 6xHis tag was expressed in ECOLI. Recombinant Human WNT1-inducible-signaling pathway protein 3 encodes amino acid residues 24-354.
• Biological Activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 38202241
• NCBI GeneID: 8838
• NCBI Accession #: NP_937882.1
• NCBI GenBank Nucleotide #: NM_198239.1
• UniProt Accession #: O95389; Q3KR29; Q5H8W4; Q6UXH6
• Molecular Weight: 41,402 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 3 isoform 3
• NCBI Official Synonym Full Names: cellular communication network factor 6
• NCBI Official Symbol: CCN6
• NCBI Official Synonym Symbols: PPD; LIBC; PPAC; WISP3; WISP-3
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 3
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP3
• UniProt Synonym Gene Names: CCN6; WISP-3

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.