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Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human Collagen XII alpha1 Polyclonal Antibody | anti-COL12A1 antibody

Collagen XII alpha1 Antibody

Gene Names
COL12A1; UCMD2; BTHLM2; COL12A1L; BA209D8.1; DJ234P15.1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
Collagen XII alpha1; Polyclonal Antibody; Collagen XII alpha1 Antibody; Collagen alpha-1(XII) chain; COCA1; anti-COL12A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Collagen XII alpha1 Antibody detects endogenous levels of total Collagen XII alpha1 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
2,987
Applicable Applications for anti-COL12A1 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen XII alpha1.
Immunogen Range
1481-1530
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of COS7 cells, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
333 kDa
NCBI Official Full Name
collagen alpha-1(XII) chain long isoform
NCBI Official Synonym Full Names
collagen type XII alpha 1 chain
NCBI Official Symbol
COL12A1
NCBI Official Synonym Symbols
UCMD2; BTHLM2; COL12A1L; BA209D8.1; DJ234P15.1
NCBI Protein Information
collagen alpha-1(XII) chain
UniProt Protein Name
Collagen alpha-1(XII) chain
Protein Family
UniProt Gene Name
COL12A1
UniProt Synonym Gene Names
COL12A1L

NCBI Description

This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.

Research Articles on COL12A1

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Product Notes

The COL12A1 col12a1 (Catalog #AAA9505711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen XII alpha1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XII alpha1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the COL12A1 col12a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XII alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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