PSPN active protein
PSPN
Gene Names
PSPN; PSP
Applications
SDS-Page
Purity
>95%, as determined by SDS-PAGE and HPLC.
Synonyms
PSPN; PSPN active protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Form/Format
Lyophilized; PBS, pH 7.2
Sequence
RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG
Sequence Length
156
Applicable Applications for PSPN active protein
SDS-PAGE
Application Notes
Recombinant PSPN migrates as an approximately 11 kDa protein under reducing conditions in SDS-PAGE.
Species
Human
Source Note
Optimized DNA sequence encoding Human PSPN mature chain was expressed in ECOLI. Recombinant Human Persephin encodes amino acid residues 22-156.
Biological Activity
Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,600 Da
NCBI Official Full Name
persephin preproprotein
NCBI Official Synonym Full Names
persephin
NCBI Official Symbol
PSPN
NCBI Official Synonym Symbols
PSP
NCBI Protein Information
persephin
UniProt Protein Name
Persephin
Protein Family
UniProt Gene Name
PSPN
UniProt Synonym Gene Names
PSP
NCBI Description
This gene encodes a secreted ligand of the GDNF (glial cell line-derived neurotrophic factor) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. This protein may play a role in cell death, and nervous system development and function. Elevated expression of this gene has been observed in oral squamous cell carcinoma. [provided by RefSeq, Aug 2016]
Uniprot Description
Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.
Research Articles on PSPN
Similar Products
Product Notes
The PSPN pspn (Catalog #AAA553634) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PSPN can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Recombinant PSPN migrates as an approximately 11 kDa protein under reducing conditions in SDS-PAGE. Researchers should empirically determine the suitability of the PSPN pspn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG . It is sometimes possible for the material contained within the vial of "PSPN, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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