IL-35 Recombinant Protein | IL35 recombinant protein

Human IL-35 Recombinant

Cat. Num. AAA553262

• Purity: >95%, as determined by SDS-PAGE
• Synonyms: IL-35; Human IL-35 Recombinant; il-35; il35; IL35 recombinant protein

• Host: HEK Cells
• Purity/Purification: >95%, as determined by SDS-PAGE
• Form/Format: Recombinant Human Interleukin-35 was lyophilized from a 0.2 um filtered PBS solution pH 7.4.
• Sequence Length: 229

• Host Note: DNA sequence encoding Human IL27B fused to DNA sequence encoding mature chain of Human IL12 p35 via linker chain and a C-terminal polyHis tag was expressed in HEK cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Recombinant Human Interleukin-35 is a monomer consisting of 645 amino acid residue, and migrates as an approximately 95 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Product Categories/Family for IL35 recombinant protein

Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 14577917
• NCBI GeneID: 10148
• NCBI Accession #: NP_005746.2
• NCBI GenBank Nucleotide #: NM_005755.2
• UniProt Accession #: Q14213; O75269; A0N0N2
• Molecular Weight: 25,396 Da
• NCBI Official Full Name: interleukin-27 subunit beta
• UniProt Protein Name: Interleukin-27 subunit beta
• UniProt Gene Name: EBI3
• UniProt Synonym Gene Names: IL27B; IL-27 subunit beta; IL-27B
• UniProt Entry Name: IL27B_HUMAN

NCBI Description

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]

Uniprot Description

IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity

Biological Process: T-helper 1 type immune response; cytokine and chemokine mediated signaling pathway; positive regulation of alpha-beta T cell proliferation; humoral immune response; positive regulation of interferon-gamma biosynthetic process