Human Interleukin-27 subunit beta ELISA Kit | EBI3 elisa kit

Human Interleukin-27 subunit beta ELISA Kit

Cat. Num. AAA762758

• Gene Names: EBI3; IL27B; IL-27B
• Reactivity: Human
• Synonyms: Interleukin-27 subunit beta; Human Interleukin-27 subunit beta ELISA Kit; EBI3/Epstein-Barr virus-induced gene 3 protein (EBV-induced gene 3 protein)/IL-27 subunit beta/IL-27B/IL-27/IL-27 p28 subunit/; EBI3 elisa kit

• Reactivity: Human
• Sequence Length: 229

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EBI3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human EBI3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EBI3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EBI3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EBI3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14577917
• NCBI GeneID: 10148
• NCBI Accession #: NP_005746.2
• NCBI GenBank Nucleotide #: NM_005755.2
• Molecular Weight: 25,396 Da
• NCBI Official Full Name: interleukin-27 subunit beta
• NCBI Official Synonym Full Names: Epstein-Barr virus induced 3
• NCBI Official Symbol: EBI3
• NCBI Official Synonym Symbols: IL27B; IL-27B
• NCBI Protein Information: interleukin-27 subunit beta
• UniProt Protein Name: Interleukin-27 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: EBI3
• UniProt Synonym Gene Names: IL27B; IL-27 subunit beta; IL-27B; EBV-induced gene 3 protein
• UniProt Entry Name: IL27B_HUMAN

NCBI Description

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]

Uniprot Description

IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-27 receptor binding; protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; humoral immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of interferon-gamma biosynthetic process; T cell proliferation; T-helper 1 type immune response