Interleukin-21 Recombinant Protein | IL-21 recombinant protein

Human Interleukin-21 Recombinant

Cat. Num. AAA553158

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-21; Human Interleukin-21 Recombinant; il21; il-21; interleukin-21; IL-21 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-21 was lyophilized from a 0.2 um filtered PBS solution pH 7.5.
• Sequence: MERIVICLMV IFLGTLVHKS SSQGQDRHMI RMRQLIDIVD QLKNYVNDLV PEFLPAPEDV ETNCEWSAFS CFQKAQLKSA NTGNNERIIN VSIKKLKRKP PSTNAGRRQK HRLTCPSCDS YEKKPPKEFL ERFKSLLQKM IHQHLSSRTH GSEDS
• Sequence Length: 153

• Host Note: Optimized DNA sequence encoding Human Interleukin-21 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human Interleukin-21 is generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated molecular mass of approximately 15 kDa. Recombinant IL-21 is a disulfide-linked homodimeric protein consisting of 132 amino acid residue subunits, and migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-21 recombinant protein

IL21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen. It inhibits proliferation stimulated by IL4 plus anti- IgM. IL21 augments stimulation of the proliferation of naive CD45RA (+) but not memory CD45R0 (+) T-cells mediated by engagement of CD3. IL21 stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL15. The Interleukin-21 receptor is closely related to human IL2 beta receptor. The receptor contains a WSXWS motif in the extracellular region, typical of type 1 cytokine receptors. The receptor is expressed on NK-cells, T- cells, and B-cell line.The functional signaling complex activates Janus kinases JAK1, JAK3, and the STAT proteins STAT1, and STAT3.

Product Categories/Family for IL-21 recombinant protein

Cytokines

References

• tlr3 ligand polyinosinic:polycytidylic acid induces il-17a and IL-21 synthesis in human th cells .J. Immunol., Oct 2009; 183: 4422 - 4431.
• IL-21 and il-10 have redundant roles but differential capacities at different stages of plasma cell generation from human germinal center b cells .J. Leukoc. Biol., Sep 2009; 10.1189/jlb.0409268.
• modulation of single-cell igg secretion frequency and rates in human memory b cells by cpg dna, cd40l, IL-21, and cell division .J. Immunol., Sep 2009; 183: 3177 - 3187.
• induction of granulysin in cd8+ t cells by IL-21 and il-15 is suppressed by human immunodeficiency virus-1 .J. Leukoc. Biol., Aug 2009; 10.1189/jlb.0409222.
• IL-21 contributes to jak3/stat3 activation and promotes cell growth in alk-positive anaplastic large cell lymphoma .Am. J. Pathol., Aug 2009; 175: 825 - 834.