MIG recombinant protein

Human MIG Recombinant

Cat. Num. AAA553131

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: MIG; Human MIG Recombinant; CXCL9; MIG recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant MIG/CXCL9 was lyophilized from 1.0 mg/ml solution in 25mM NaCl 10mM PB pH 7.0.
• Sequence: MKKSGVLFLL GIILLVLIGV QGTPVVRKGR CSCISTNQGT IHLQSLKDLK QFAPSPSCEK IEIIATLKNG VQTCLNPDSA DVKELIKKWE KQVSQKKKQK NGKKHQKKKV LKVRKSQRSR QKKTT
• Sequence Length: 125

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human MIG mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human MIG (CXCL9) is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 11 kDa.; ; Recombinant MIG (CXCL9) is a disulfide-linked monomeric protein consisting of 104 amino acid residue subunits, migrates as an approximately 11kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for MIG recombinant protein

MIG (CXCL9) protein belongs to the family of chemotactic cytokines known as Chemokines. The synthesis of MIG (CXCL9) is specifically induced in macrophages and in other cells by IFN-gamma.Human neutrophils produce mig in response to IFN-gamma in combination with either TNF- alpha or bacterial lipopolysaccharides and this response is blocked by IL-10 and IL-4.mig is a chemoattractant for stimulated but not resting T-cells,however it is not active on neutrophils or monocytes.

Product Categories/Family for MIG recombinant protein

Cytokines

References

• sufa of the opportunistic pathogen finegoldia magna modulates actions of the antibacterial chemokine mig/CXCL9, promoting bacterial survival during epithelial inflammation .J. Biol. Chem., Jul 2009; 10.1074/jbc.M109.025957.
• monokine induced by interferon- (mig/CXCL9) is derived from both donor and recipient sources during rejection of class ii major histocompatibility complex disparate skin allografts .Am. J. Pathol., Jun 2009; 174: 2172 - 2181.
• monokine induced by interferon (ifn) (CXCL9) and ifn inducible t-cell -chemoattractant (cxcl11) involvement in graves’ disease and ophthalmopathy: modulation by peroxisome proliferator-activated receptor- agonists .J. Clin. Endocrinol. Metab., May 2009; 94: 1803 - 1809.
• role of t cell chemoattractants cxcl10/ip10 and CXCL9/mig in high risk corneal allograft transplantation .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1977.
• regulation of CXCL9/10/11 in oral keratinocytes and fibroblasts .Journal of Dental Research, Dec 2008; 87: 1160 - 1165.