Typical Testing Data/Standard Curve (for reference only)
Porcine Chemokine C-X-C-Motif Ligand 9 ELISA Kit | CXCL9 elisa kit
Porcine Chemokine C-X-C-Motif Ligand 9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CXCL9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCL9 antibody and an CXCL9-HRP conjugate. The assay sample and buffer are incubated together with CXCL9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL9 concentration since CXCL9 from samples and CXCL9-HRP conjugate compete for the anti-CXCL9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL9 from the sample, fewer sites are left to bind CXCL9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The function of this gene has not been specifically defined; however, it is thought to be involved in T cell trafficking. This gene has been localized to 4q21 with INP10, which is also a member of the chemokine family of cytokines. [provided by RefSeq, Jul 2008]
Uniprot Description
CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity
Biological Process: positive regulation of leukocyte chemotaxis; defense response; response to lipopolysaccharide; positive regulation of cAMP metabolic process; signal transduction; chemotaxis; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus
Research Articles on CXCL9
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Product Notes
The Porcine CXCL9 cxcl9 (Catalog #AAA749695) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA749695 ELISA Kit recognizes Porcine CXCL9. It is sometimes possible for the material contained within the vial of "Chemokine C-X-C-Motif Ligand 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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