Interleukin-1 alpha Recombinant Protein | IL-1 recombinant protein

Mouse Interleukin-1 alpha Recombinant

Cat. Num. AAA553101

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-1 alpha; Mouse Interleukin-1 alpha Recombinant; il-1 alpha; il1a; il1; interleukin-1; il-1a; IL-1 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse Interleukin-1 alpha was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MAKVPDLFED LKNCYSENED YSSAIDHLSL NQKSFYDASY GSLHETCTDQ FVSLRTSETS KMSNFTFKES RVTVSATSSN GKILKKRRLS FSETFTEDDL QSITHDLEET IQPRSAPYTY QSDLRYKLMK LVRQKFVMND SLNQTIYQDV DKHYLSTTWL NDLQQEVKFD MYAYSSGGDD SKYPVTLKIS DSQLFVSAQG EDQPVLLKEL PETPKLITGS ETDLIFFWKS INSKNYFTSA AYPELFIATK EQSRVHLARG LPSMTDFQIS
• Sequence Length: 270

• Domain: IL1
• Host Note: Optimized DNA sequence encoding Mouse Interleukin-1 alpha mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Mature Mouse IL-1 alpha, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 18 kDa. ; ; Recombinant IL-1a is a disulfide-linked monomer protein consisting of 157 amino acid residue subunits and migrates as an approximately 18 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-1 recombinant protein

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

Product Categories/Family for IL-1 recombinant protein

Cytokines

References

• interleukin-1a (IL-1a) promotes angiogenesis in vivo via vegfr-2 pathway by inducing inflammatory cell vegf synthesis and secretion .FASEB J, Jul 2002; 10.1096/fj.02-0134fje.
• polymorphisms in the IL-1a gene are correlated with levels of interleukin-1 protein in gingival crevicular fluid of teeth with severe periodontal disease .Journal of Dental Research, Nov 2000; 79: 1864 - 1869.