S100 Calcium Binding Protein A4 Recombinant Protein | S100-A4 recombinant protein

Human S100 Calcium Binding Protein A4 Recombinant

Cat. Num. AAA553027

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: S100 Calcium Binding Protein A4; Human S100 Calcium Binding Protein A4 Recombinant; s100a4; s100-a4; capl; S100-A4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant S100-A4 was lyophilized from a 0.2 um filtered PBS solution and 1 mM DTT.
• Sequence: MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K
• Sequence Length: 101

• Domain: EF_HAND
• Host Note: Optimized DNA sequence encoding Human S100A4 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human S100A4, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 12 kDa. Recombinant s100-A4 is a disulfide-linked homodimer protein consisting of 332 amino acid residue subunits (containing a C-terminal IgG1 Fc tag), and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for S100-A4 recombinant protein

S100A4 is a calcium-binding protein related to the S100 family of proteins. It is known also as a growth- related protein, CAPL. It is expressed at high levels in different mouse metastatic cells and in normal tissues (thymus, spleen) and activated macrophages, T- cells, neutrophils, monocytes, macrophages.

Product Categories/Family for S100-A4 recombinant protein

Cytokines

References

• S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho–extracellular signal-regulated kinase and chloride intracellular channel 4 .Circ. Res., Sep 2009; 105: 639 - 647.
• autoimmune pancreatitis results from loss of tgfβ signalling in S100A4-positive dendritic cells .Gut, Sep 2009; 58: 1267 - 1274.
• opposite roles of metastasin (S100A4) in two potentially tumoricidal mechanisms involving human lymphocyte protein tag7 and hsp70 .PNAS, Aug 2009; 106: 13963 - 13967.
• significance of S100A4 as a prognostic marker of lung squamous cell carcinoma .Anticancer Res, Jul 2009; 29: 2547 - 2554.
• integrin 6β4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin .J. Biol. Chem., Jan 2009; 284: 1484 - 1494.