Rabbit BMP-4 Polyclonal Antibody | anti-BMP-4 antibody

Rabbit anti BMP-4 Polyclonal

Cat. Num. AAA551014

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Serum IgG fraction was purified by Protein G affinity chromatography.
• Synonyms: BMP-4; Polyclonal Antibody; Rabbit anti BMP-4 Polyclonal; Rabbit anti BMP-4 Polyclonal purified; Bone Morphogenetic protein ; bmp-4; bmp4; polyclonal; PAb; anti-BMP-4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum IgG fraction was purified by Protein G affinity chromatography.
• Form/Format: Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
• Sequence Length: 408

• Applicable Applications for anti-BMP-4 antibody: Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
• Application Notes: ELISA: Use at a concentration range of 1-2ug/ml.; Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.; IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
• Source Note: Polyclonal BMP-4 antibody was produced from sera of rabbits immunized with synthetic protein containing BMP-4 antigenic determinants.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
• Preparation and Storage: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.; Shelf Life: >12 months

Related Product Information for anti-BMP-4 antibody

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP4 and BMP7 have been shown to be involved also in the differentiation of sympathetic neurons. They enhance the formation of adrenergic sympathetic neurons in cultures of neural crest cells.

Product Categories/Family for anti-BMP-4 antibody

Polyclonal

References

• smad, pi3k/akt, and wnt-dependent signaling pathways are involved in BMP-4-induced esc self-renewal .Stem Cells, Aug 2009; 27: 1858 - 1868.
• molecular interaction of BMP-4, tgf-β, and estrogens in lactotrophs: impact on the prl promoter .Mol. Endocrinol., Jul 2009; 23: 1102 - 1114.
• suppression of vegf expression and lesion size in laser-induced cnv in a mouse model overexpressing BMP-4 .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1193.
• altered shear stress stimulates upregulation of endothelial vcam-1 and icam-1 in a BMP-4– and tgf-β1–dependent pathway .Arterioscler Thromb Vasc Biol, Feb 2009; 29: 254 - 260.
• the bone morphogenetic protein (bmp) -4 is involved in the regulation of human megakaryocytic differentiation during thrombopoietin signaling. .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1339.