SOCS3 positive control

SOCS3 Antibody Western Blot Positive Control

Cat. Num. AAA543274

• Gene Names: SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• Applications: Western Blot
• Synonyms: SOCS3; SOCS3 Antibody Western Blot Positive Control; ATOD4; CIS 3; Cish3; Cytokine induced SH2 protein 3; E2a Pbx1 target gene in fibroblasts 10; EF 10; SOCS 3; SSI3; STAT induced STAT inhibitor 3; Suppressor of cytokine signaling 3 antibody; SOCS3 positive control

• Form/Format: Western Blot Positive Control
• Sequence Length: 225

• Applicable Applications for SOCS3 positive control: Western Blot (WB)
• Application Notes: Western Blot: 1:500
• Immunogen: Purified Protein
• Expression: Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
• Molecular Function: Protein modification; protein ubiquitination.
• Structure: Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR (By similarity). Interacts with BCL10; this interaction may interfere with BCL10-binding with PELI2.
• Subcellular Location: Cytoplasm
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for SOCS3 positive control

Western Blot Positive Control SOCS3, partial Antibody
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.

NCBI and Uniprot Product Information

• NCBI GI #: 49456349
• NCBI GeneID: 9021
• UniProt Accession #: Q6FI39; O14543
• Molecular Weight: 24,770 Da
• NCBI Official Full Name: SOCS3, partial
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: SOCS3
• NCBI Official Synonym Symbols: CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• NCBI Protein Information: suppressor of cytokine signaling 3
• UniProt Protein Name: SOCS3 protein
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS3
• UniProt Entry Name: Q6FI39_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]