KAP1 Polyclonal Antibody | anti-KAP1 antibody

Anti-KAP1 Antibody

Cat. Num. AAA177753

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: KAP1; Polyclonal Antibody; Anti-KAP1 Antibody; Transcription intermediary factor 1-beta; E3 SUMO protein ligase TRIM28; E3 SUMO-protein ligase TRIM28; FLJ29029; KAP 1; KAP-1; KRAB associated protein 1; KRAB interacting protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP 1; KRIP-1; KRIP1; Nuclear corepressor KAP 1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1 beta; TIF1-beta; TIF1B; TIF1B_HUMAN; Transcription intermediary factor 1 beta; Trim28; Tripartite motif containing 28; tripartite motif containing protein 28; Tripartite motif-containing protein 28; tripartite motif containing 28; anti-KAP1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 835

• Applicable Applications for anti-KAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-KAP1 Picoband antibody, MBS177753, Western blottingAll lanes: Anti KAP1 (MBS177753) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: SKOV Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 105KDObserved bind size: 105KD )

Immunohistochemistry (IHC)

(Anti-KAP1 Picoband antibody, MBS177753, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-KAP1 Picoband antibody, MBS177753, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-KAP1 Picoband antibody, MBS177753, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-KAP1 antibody

Description: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1beta (TIF1beta) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

References

1. Capili AD, Schultz DC, RauscherIII FJ, Borden KL (Jan 2001). "Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains". The EMBO Journal 20 (1-2): 165-77. 2. Iyengar, Sushma; Farnham, Peggy (2011-07-29). "KAP1 Protein: An Enigmatic Master Regulator of the Genome". The Journal of Biological Chemistry 286. 3. Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (May 2001). "The tripartite motif family identifies cell compartments". The EMBO Journal 20 (9): 2140-51.

NCBI and Uniprot Product Information

• NCBI GI #: 5032179
• NCBI GeneID: 10155
• NCBI Accession #: NP_005753.1
• NCBI GenBank Nucleotide #: NM_005762.2
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 79,474 Da
• NCBI Official Full Name: transcription intermediary factor 1-beta
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta
• UniProt Protein Name: Transcription intermediary factor 1-beta
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
• UniProt Entry Name: TIF1B_HUMAN

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: Kinase, protein; Transcription, coactivator/corepressor; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Protein kinase, atypical; Nuclear receptor co-regulator; Ligase; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus

Molecular Function: DNA binding; ligase activity; protein binding; protein kinase activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: chromatin modification; convergent extension involved in axis elongation; DNA methylation during embryonic development; DNA repair; embryo implantation; epithelial to mesenchymal transition; innate immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of DNA repair; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein oligomerization; protein sumoylation; protein ubiquitination; Ras protein signal transduction; transcription initiation from RNA polymerase II promoter