Western Blot (WB)
Rabbit Phospho-PDE4A Polyclonal Antibody | anti-PDE4A antibody
Phospho-PDE4A Antibody
Reactivity
Human, Mouse, Rabbit, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
Phospho-PDE4A; Polyclonal Antibody; Phospho-PDE4A Antibody; cAMP specific 3'; 5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific 3' 5' cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; cyclic AMP phosphodiesterase; DPDE 3; FLJ97311; HSPDE4D; PDE 43; Pde4d; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific; Phosphodiesterase E3 dunce homolog Drosophila; STRK 1; STRK1 antibody; anti-PDE4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Specificity
This antibody detects all known long-form variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D.
Form/Format
Affinity Purified
Concentration
0.57 ug/ul in antibody stabilization buffer (varies by lot)
Applicable Applications for anti-PDE4A antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500
Immunogen
Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue.
Expression
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Modification
Phosphorylated
Molecular Function
Hydrolase, 3', 5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Structure
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Subcellular Location
Apical cell membrane, Cytoplasm, Membrane
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-PDE4A antibody
Affinity Purified Phosphorylated Phosphodiesterase 4A (S140) Antibody
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Product Categories/Family for anti-PDE4A antibody
Similar Products
Product Notes
The PDE4A (Catalog #AAA540102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PDE4A Antibody reacts with Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PDE4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:150 Western Blot: 1:500. Researchers should empirically determine the suitability of the PDE4A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PDE4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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