SPG21 recombinant protein

SPG21, 1-308aa, Human, His tag, E Coli

Cat. Num. AAA203178

• Gene Names: SPG21; MAST; ACP33; GL010; BM-019
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SPG21; 1-308aa; Human; His tag; E Coli; Spastic paraplegia 21 isoform a; ACP33; BM-019; GL010; Maspardin; MAST; Spastic paraplegia 21 isoform a Acid cluster protein 33; BM019; Spastic paraplegia 21 autosomal recessive Mast syndrome protein; SPG21.; SPG21 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0)
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS LGISQEEQ
• Sequence Length: 308

• Applicable Applications for SPG21 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SPG21 recombinant protein

Spastic paraplegia 21 (SPG21), also known as Maspardin, binds to the hydrophobic C-terminal amino acids of CD4 and may play a role as a negative regulatory factor in CD4-depenent T-cell activation. This protein is widely expressed in various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in SPG21 cause Mast syndrome, an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum and white matter abnormalities. Recombinant human SPG21 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for SPG21 recombinant protein

Neuroscience

References

Simpson MA., et al. (2003). Am J Hum Genet. 73 (5):1147-56.; Zeitlmann L., et al. (2001). J Biol Chem. 276 (12):9123-32.;

NCBI and Uniprot Product Information

• NCBI GI #: 7706174
• NCBI GeneID: 51324
• NCBI Accession #: NP_057714
• NCBI GenBank Nucleotide #: NM_016630.6
• UniProt Accession #: Q9NZD8; Q6ZMB6; B4DW44
• Molecular Weight: 37.1 kDa (328aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: maspardin isoform a
• NCBI Official Synonym Full Names: SPG21, maspardin
• NCBI Official Symbol: SPG21
• NCBI Official Synonym Symbols: MAST; ACP33; GL010; BM-019
• NCBI Protein Information: maspardin
• UniProt Protein Name: Maspardin
• Protein Family: Maspardin
• UniProt Gene Name: SPG21
• UniProt Synonym Gene Names: ACP33

NCBI Description

The protein encoded by this gene binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation. The interaction with CD4 is mediated by the noncatalytic alpha/beta hydrolase fold domain of this protein. It is thus proposed that this gene product modulates the stimulatory activity of CD4. Mutations in this gene are associated with autosomal recessive spastic paraplegia 21 (SPG21), also known as mast syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

May play a role as a negative regulatory factor in CD4-dependent T-cell activation.