PDGF AB active protein
PDGF AB protein
Applications
User optimized
Purity
> 98% pure
Synonyms
PDGF AB; PDGF AB protein; ODGF protein; Glioma-derived growth factor protein; PCGF protein; GDGF protein; Osteosarcoma-derived Growth Factor protein; Platelet-Derived Growth Factor-AB protein; PDGF AB active protein
Host
Human
Purity/Purification
> 98% pure
Form/Format
Lyophilized from 3mM Tris, pH 8.0. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml.
Sequence
Region of PDGF AB protein corresponding to amino acids MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPTbeta chain MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT.
Applicable Applications for PDGF AB active protein
User optimized
Protein Type
Recombinant
Biological Significance
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms.
Bioactivity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is approximately 1 ng/ml, corresponding to a specific activity of approximately 1 x 10^6 units/mg.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for PDGF AB active protein
Purified recombinant Human PDGF AB protein
Product Categories/Family for PDGF AB active protein
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Product Notes
The PDGF AB (Catalog #AAA537870) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDGF AB can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the PDGF AB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Region of PDGF AB protein correspond ing to amino acids MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPTbeta chain MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT. It is sometimes possible for the material contained within the vial of "PDGF AB, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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