Rabbit anti-Human PYCR2 Polyclonal Antibody | anti-PYCR2 antibody

PYCR2 (Pyrroline-5-carboxylate Reductase 2, P5C Reductase 2, P5CR 2, P5CR2) APC

Cat. Num. AAA6391595

• Gene Names: PYCR2; HLD10; P5CR2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PYCR2; Polyclonal Antibody; PYCR2 (Pyrroline-5-carboxylate Reductase 2; P5C Reductase 2; P5CR 2; P5CR2) APC; EC=1.5.1.2; anti-PYCR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PYCR2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 320

• Applicable Applications for anti-PYCR2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PYCR2, aa1-320 (NP_037460.2).
• Immunogen Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PYCR2 rabbit polyclonal antibody. Western Blot analysis of PYCR2 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of PYCR2 expression in transfected 293T cell line by PYCR2 polyclonal antibody. Lane 1: PYCR2 transfected lysate (33.6kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-PYCR2 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 21361454
• NCBI GeneID: 29920
• NCBI Accession #: NP_037460.2
• NCBI GenBank Nucleotide #: NM_013328.2
• UniProt Accession #: Q96C36
• NCBI Official Full Name: pyrroline-5-carboxylate reductase 2 isoform 1
• NCBI Official Synonym Full Names: pyrroline-5-carboxylate reductase 2
• NCBI Official Symbol: PYCR2
• NCBI Official Synonym Symbols: HLD10; P5CR2
• NCBI Protein Information: pyrroline-5-carboxylate reductase 2
• UniProt Protein Name: Pyrroline-5-carboxylate reductase 2
• Protein Family: Pyrroline-5-carboxylate reductase
• UniProt Gene Name: PYCR2
• UniProt Synonym Gene Names: P5C reductase 2; P5CR 2
• UniProt Entry Name: P5CR2_HUMAN

NCBI Description

This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

PYCR2: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Belongs to the pyrroline-5-carboxylate reductase family.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.5.1.2

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: cytoplasm

Molecular Function: pyrroline-5-carboxylate reductase activity

Disease: Leukodystrophy, Hypomyelinating, 10