Mouse GATA2 Monoclonal Antibody | anti-GATA2 antibody

GATA2 antibody

Cat. Num. AAA533755

• Gene Names: GATA2; DCML; IMD21; NFE1B; MONOMAC
• Applications: Dot Blot, Immunocytochemistry
• Purity: GATA2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Synonyms: GATA2; Monoclonal Antibody; GATA2 antibody; Monoclonal GATA2; Anti-GATA2; GATA-2; MGC2306; GATA 2; GATA binding protein 2; NFE1B; anti-GATA2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 353C5a
• Specificity: Human GATA2
• Purity/Purification: GATA2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Form/Format: GATA2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4, 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
• Concentration: 100 ug/ml (varies by lot)
• Sequence Length: 466

• Applicable Applications for anti-GATA2 antibody: Dot Blot (DB), Immunocytochemistry (ICC)
• Application Notes: To be optimized by the end user.
• Biological Significance: The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. GATA1 is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation. GATA3 expression is restricted to T-lymphoid cells and some nonhematopoietic cell types, including embryonic stem cells.
• Cross-Reactivity: Human. Other species not studied
• Biohazard Information: This antibody solution contains sodium azide (NaN) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice.
• Immunogen: GATA2 antibody was raised in mouse using recombinant Human Gata Binding Protein 2
• Preparation and Storage: Store at 2-8 degree C for up to one year. We recommend long term storage at -20 degree C. Avoid repeated freezing and thawing.

Western Blot (WB)

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Related Product Information for anti-GATA2 antibody

Mouse monoclonal GATA2 antibody

Product Categories/Family for anti-GATA2 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 30410878
• NCBI GeneID: 2624
• UniProt Accession #: P23769; Q53YE0; Q96BH0; Q96BH8; Q9BUJ6; D3DNB3
• Molecular Weight: 49,106 Da
• NCBI Official Full Name: GATA2 protein
• NCBI Official Synonym Full Names: GATA binding protein 2
• NCBI Official Symbol: GATA2
• NCBI Official Synonym Symbols: DCML; IMD21; NFE1B; MONOMAC
• NCBI Protein Information: endothelial transcription factor GATA-2
• UniProt Protein Name: Endothelial transcription factor GATA-2
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA2
• UniProt Entry Name: GATA2_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; protein complex

Molecular Function: protein binding; zinc ion binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: embryonic placenta development; inner ear morphogenesis; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of erythrocyte differentiation; cell maturation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; negative regulation of fat cell differentiation; phagocytosis; cell differentiation in hindbrain; positive regulation of angiogenesis; negative regulation of macrophage differentiation; homeostasis of number of cells within a tissue; commitment of a neuronal cell to a specific type of neuron in the forebrain; pituitary gland development; ventral spinal cord interneuron differentiation; positive regulation of phagocytosis; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; blood coagulation; urogenital system development; central nervous system neuron development

Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome