Western Blot (WB)
(Western blot analysis using p27Kip1 antibody; Lane1: The extract from A2780 cells; Lane2: The extract from COLO205 cells; Lane3: The extract from COLO205 cells using antibody preincubated with synthesized peptide.)
Rabbit p27Kip1 Polyclonal Antibody | anti-p27Kip1 antibody
p27Kip1 Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
p27Kip1; Polyclonal Antibody; p27Kip1 Antibody; Rabbit Polyclonal p27Kip1 Antibody; Polyclonal p27Kip1 antibody; Anti-p27Kip1 antibody; MDA-6 antibody; MDA6 antibody; melanoma differentiation associated protein 6 antibody; Melanoma differentiation-associated protein 6 antibody; p21 antibody; p21CIP1 antibody; p21Cip1/Waf1 antibody; PIC1 antibody; SDI1 antibody; WAF1 antibody; wild-type p53-activated fragment 1 antibody; CAP20 antibody; CDK-interacting protein 1 antibody; CDK-interaction protein 1 antibody; CDKN1 antibody; CDKN1A antibody; CDN1A antibody; CIP1 antibody; Cyclin-dependent kinase inhibitor 1 antibody; cyclin-dependent kinase inhibitor 1A (p21 Cip1) antibody; DNA synthesis inhibitor antibody; anti-p27Kip1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
p27Kip1 antibody detects endogenous levels of total p27Kip1 protein
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
9
Applicable Applications for anti-p27Kip1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
p27Kip1 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-SP-P-S) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis using p27Kip1 antibody; Lane1: The extract from A2780 cells; Lane2: The extract from COLO205 cells; Lane3: The extract from COLO205 cells using antibody preincubated with synthesized peptide.)
Western Blot (WB)
(Western blot analysis using p27Kip1 antibody; Lane1: The extract from A2780 cells; Lane2: The extract from COLO205 cells; Lane3: The extract from COLO205 cells using antibody preincubated with synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using p27Kip1 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using p27Kip1 antibody)
Related Product Information for anti-p27Kip1 antibody
p27Kip1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Product Categories/Family for anti-p27Kip1 antibody
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Research Articles on p27Kip1
Similar Products
Product Notes
The p27Kip1 (Catalog #AAA5313915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p27Kip1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the p27Kip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p27Kip1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
