Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4EBP1 antibody (Thr45))
Rabbit anti-Mouse, Rat 4EBP1 Polyclonal Antibody | anti-4EBP1 antibody
4EBP1 Antibody (Thr45)
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
4EBP1; Polyclonal Antibody; 4EBP1 Antibody (Thr45); Rabbit Polyclonal 4EBP1 Antibody (Thr45); Polyclonal 4EBP1 antibody; Anti-4EBP1 antibody; 4EBP1 antibody; EIF4EBP1 antibody; Eukaryotic translation initiation factor 4E binding protein 1 antibody; Insulin-stimulated EIF-4E binding protein PHAS-I antibody; P/OKCL.6 antibody; PHAS-1 antibody; PHAS-I antibody; anti-4EBP1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
4EBP1 antibody (Thr45) detects endogenous levels of 4EBP1 only when phosphorylated at threonine 45. The antibody will detect species specific phosphorylation as follows: Human: Thr45 or Thr46, Mouse: Thr44, Rat: Thr44
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
129
Applicable Applications for anti-4EBP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
4EBP1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G) as the immunogen.
Cross-Reactivity
Mouse, Rat. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4EBP1 antibody (Thr45))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4EBP1 antibody (Thr45))
Western Blot (WB)
(Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 antibody and 4EBP1 antibody (Thr45) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min), using 4E-BP1 antibody and 4EBP1 antibody (Thr45) (Lanes 3 and 4))
Related Product Information for anti-4EBP1 antibody
4EBP1 is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Product Categories/Family for anti-4EBP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
4EBP1, partial
Similar Products
Product Notes
The 4EBP1 (Catalog #AAA5313649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 4EBP1 Antibody (Thr45) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 4EBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the 4EBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4EBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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