Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Rabbit 4E-BP1 Polyclonal Antibody | anti-4E-BP1 antibody
4E-BP1 antibody
Gene Names
EIF4EBP1; 4E-BP1
Applications
Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
4E-BP1; Polyclonal Antibody; 4E-BP1 antibody; Rabbit polyclonal 4E-BP1 antibody; Polyclonal 4E-BP1 antibody; Anti-4E-BP1 antibody; Eukaryotic translation initiation factor 4E-binding protein 1 antibody; E-BP1-4 antibody; E-BP1 4; E-BP1-4; Phospho Thr45 antibody; E-BP1 4 antibody; anti-4E-BP1 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
118
Applicable Applications for anti-4E-BP1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
WB: 1:500-1:1000
Immunogen
4E-BP1 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from Human 4E-BP1 as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Western Blot (WB)
(Western Blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr45) antibody and the same antibody preincubated with blocking peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr45) antibody and the same antibody preincubated with blocking peptide.)
Related Product Information for anti-4E-BP1 antibody
The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Product Categories/Family for anti-4E-BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
12,489 Da
NCBI Official Full Name
4E-BP1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
4E-BP1
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
4E-BP1
UniProt Gene Name
4E-BP1
Research Articles on 4E-BP1
Similar Products
Product Notes
The 4E-BP1 4e-bp1 (Catalog #AAA5309581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the 4E-BP1 4e-bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
