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Western Blot (WB) (Western Blot analysis of K562 cell Lysate with anti-HKI antibody)

Rabbit HK1 Polyclonal Antibody | anti-HK1 antibody

HK1 Antibody

Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
HK1; Polyclonal Antibody; HK1 Antibody; Rabbit Polyclonal HK1 Antibody; Polyclonal HK1 antibody; Anti-HK1 antibody; brain form hexokinase antibody; glycolytic enzyme antibody; hexokinase 1 antibody; Hexokinase 1 isoform HKI variant antibody; hexokinase type I antibody; hexokinase-1 antibody; HK I antibody; HK1 antibody; HK1-ta antibody; HK1-tb antibody; HK1-tc antibody; HKI antibody; HXK1 antibody; anti-HK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in liquid format, PBS buffer, pH 7.4, 150mM NaCl with added preservative 0.02% Sodium Azide and 50% Glycerol
Sequence Length
1042
Applicable Applications for anti-HK1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:20,000-1:80,000
WB: 1:1,000-1:2,000
IHC: 1:100-1:500
Immunogen
HK1 antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human HK1 as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of K562 cell Lysate with anti-HKI antibody)

Western Blot (WB) (Western Blot analysis of K562 cell Lysate with anti-HKI antibody)

Immunohistochemistry (IHC)

(Immunohistochemical staining of formalin-fixed paraffin-embedded fetal sketal muscle showing cytoplasmic staining with anti-HKI antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemical staining of formalin-fixed paraffin-embedded fetal sketal muscle showing cytoplasmic staining with anti-HKI antibody at a dilution of 1/100)
Related Product Information for anti-HK1 antibody
HK1 is a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose.
Product Categories/Family for anti-HK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
488
NCBI Official Full Name
HK1
NCBI Official Synonym Full Names
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
NCBI Official Symbol
ATP2A2
NCBI Official Synonym Symbols
DD; DAR; ATP2B; SERCA2
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Protein Family

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Research Articles on HK1

Similar Products

Product Notes

The HK1 (Catalog #AAA5313351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:20,000-1:80,000 WB: 1:1,000-1:2,000 IHC: 1:100-1:500. Researchers should empirically determine the suitability of the HK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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