Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.)
Rabbit PDE4D Polyclonal Antibody | anti-PDE4D antibody
PDE4D Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
PDE4D; Polyclonal Antibody; PDE4D Antibody; Affinity Purified Rabbit Polyclonal PDE4D Antibody; Polyclonal PDE4D antibody; Anti-PDE4D antibody; PDED 4 antibody; PDE4DN2 antibody; DPDE3 antibody; cAMP-specific phosphodiesterase PDE4D6 antibody; PDED-4 antibody; phosphodiesterase 4D cAMP-specific (phosphodiesterase E3 dunce homolog Drosophila) antibody; PDE4D antibody; FLJ97311 antibody; PDED-4; DKFZp686M11213 antibody; PDED 4; HSPDE4D antibody; STRK1 antibody; anti-PDE4D antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.3 mg/ml (varies by lot)
Sequence Length
147
Applicable Applications for anti-PDE4D antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:1000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Immunogen
PDE4D antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 746 and 809 of PDE4D
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: HeLa) using a 7.5% SDS PAGE gel and PDE4D antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431; B: HeLa) using a 7.5% SDS PAGE gel and PDE4D antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded H441 xenograft using PDE4D antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded H441 xenograft using PDE4D antibody at a dilution of 1:100)
Related Product Information for anti-PDE4D antibody
The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells.
Product Categories/Family for anti-PDE4D antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
76 kDa
NCBI Official Full Name
PDE4D, partial
Protein Family
Similar Products
Product Notes
The PDE4D (Catalog #AAA5311965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDE4D can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:1000 ICC: 1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PDE4D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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