Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Rabbit anti-Human PDE4D Polyclonal Antibody | anti-PDE4D antibody
PDE4D antibody
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D antibody at 1: 100 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D antibody at 1: 100 dilution.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 500.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 500.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Uniprot Description
PDE4D: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Inhibited by rolipram. Activated by phosphatidic acid. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 12 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase
Chromosomal Location of Human Ortholog: 5q12
Cellular Component: Golgi apparatus; centrosome; membrane; myofibril; apical plasma membrane; voltage-gated calcium channel complex; cytosol
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; enzyme binding; metal ion binding; ubiquitin protein ligase binding; beta-2 adrenergic receptor binding; drug binding; cAMP binding; SH3 domain binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; ATPase binding
Biological Process: neutrophil chemotaxis; negative regulation of heart contraction; regulation of heart rate; positive regulation of smooth muscle cell proliferation; multicellular organism growth; cAMP catabolic process; negative regulation of peptidyl-serine phosphorylation; positive regulation of smooth muscle cell migration; T cell receptor signaling pathway; memory; positive regulation of interferon-gamma production; cAMP-mediated signaling; smooth muscle contraction; cellular protein complex assembly; positive regulation of interleukin-2 production; regulation of receptor activity; positive regulation of interleukin-5 production; regulation of G-protein coupled receptor protein signaling pathway; aging; lung development
Research Articles on PDE4D
Similar Products
Product Notes
The PDE4D pde4d (Catalog #AAA9408134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE4D antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE4D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the PDE4D pde4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
