Rabbit anti-Human PDE4D Polyclonal Antibody | anti-PDE4D antibody

PDE4D antibody

Cat. Num. AAA9408134

• Gene Names: PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: PDE4D; Polyclonal Antibody; PDE4D antibody; DPDE3; STRK1; HSPDE4D; PDE4DN2; anti-PDE4D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 673

• Applicable Applications for anti-PDE4D antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250; Immunofluorescence: 1:100-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide contain a sequence corresponding to a region within amino acids 610 and 673 of PDE4D
• Target Name: PDE4D
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 500.)

Related Product Information for anti-PDE4D antibody

The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM]

Product Categories/Family for anti-PDE4D antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 32306513
• NCBI GeneID: 5144
• NCBI Accession #: NP_006194
• NCBI GenBank Nucleotide #: NM_006203.4
• Molecular Weight: 24,429 Da
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform PDE4D3
• NCBI Official Synonym Full Names: phosphodiesterase 4D, cAMP-specific
• NCBI Official Symbol: PDE4D
• NCBI Official Synonym Symbols: DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4D
• UniProt Synonym Gene Names: DPDE3
• UniProt Entry Name: PDE4D_HUMAN

NCBI Description

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]

Uniprot Description

PDE4D: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Inhibited by rolipram. Activated by phosphatidic acid. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: Golgi apparatus; centrosome; membrane; myofibril; apical plasma membrane; voltage-gated calcium channel complex; cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; enzyme binding; metal ion binding; ubiquitin protein ligase binding; beta-2 adrenergic receptor binding; drug binding; cAMP binding; SH3 domain binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; ATPase binding

Biological Process: neutrophil chemotaxis; negative regulation of heart contraction; regulation of heart rate; positive regulation of smooth muscle cell proliferation; multicellular organism growth; cAMP catabolic process; negative regulation of peptidyl-serine phosphorylation; positive regulation of smooth muscle cell migration; T cell receptor signaling pathway; memory; positive regulation of interferon-gamma production; cAMP-mediated signaling; smooth muscle contraction; cellular protein complex assembly; positive regulation of interleukin-2 production; regulation of receptor activity; positive regulation of interleukin-5 production; regulation of G-protein coupled receptor protein signaling pathway; aging; lung development