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Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 K Isotype Control (open histogram) or 0.25 ug of CD24 antibody) (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)

Mouse anti-Human CD24 Monoclonal Antibody | anti-CD24 antibody

CD24 antibody

Gene Names
CD24; CD24A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
CD24; Monoclonal Antibody; CD24 antibody; Mouse monoclonal CD24 antibody; Monoclonal CD24 antibody; Anti-CD24 antibody; Heat Stable Antigen antibody; CD-24 antibody; HAS antibody; CD 24; BA-1 antibody; CD 24 antibody; CD-24; anti-CD24 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SN3
Specificity
Human
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
80
Applicable Applications for anti-CD24 antibody
Flow Cytometry (FC/FACS), Functional Assay, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
FC/FACS: = 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 K Isotype Control (open histogram) or 0.25 ug of CD24 antibody) (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)

Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 K Isotype Control (open histogram) or 0.25 ug of CD24 antibody) (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD24 antibody
The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen. This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin binds to CD24.
Product Categories/Family for anti-CD24 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
12,968 Da
NCBI Official Full Name
CD24
NCBI Official Synonym Full Names
CD24 molecule
NCBI Official Symbol
CD24
NCBI Official Synonym Symbols
CD24A
NCBI Protein Information
signal transducer CD24
UniProt Protein Name
Signal transducer CD24
Protein Family
UniProt Gene Name
CD24
UniProt Synonym Gene Names
CD24A

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed:11313396). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity ().

Research Articles on CD24

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Product Notes

The CD24 cd24 (Catalog #AAA5309238) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD24 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD24 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). FC/FACS: = 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the CD24 cd24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD24, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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