Mouse anti-Human CD24 Monoclonal Antibody | anti-CD24 antibody

anti-human CD24-Purified (with Antibiotics)

Cat. Num. AAA666632

• Gene Names: CD24; CD24A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified (with Antibiotics)
• Synonyms: CD24; Monoclonal Antibody; anti-human CD24-Purified (with Antibiotics); Monoclonal anti-human CD24; anti-CD24 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: BA-1
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 80

• Applicable Applications for anti-CD24 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD24 at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.
• Production: Antibody from (low FBS containing) tissue culture supernatant was purified to 95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin) using size exclusion chromatography
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD24
• Immunogen: Human B lymphocyte tumor cells: NALM-6-M1
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD24 antibody

Information: Human CD24 is a glycosyl phosphatidyl inositol (GPI) anchored surface protein found on B cells during multiple stages of development from precursor to the onset of plasma cell differentiation. Antibody BA-1 recognizes a sialic acid-dependent epitope of human CD24 of approximately 35/45 kd.

References

C.S. Abramson, et al, (1981) J Immunol 126: 83-88. H. Mehmet, et al, (1990) Clin Exp Immunol 81: 489-495. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 82-84. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 539-543.

NCBI and Uniprot Product Information

• NCBI GI #: 209405388
• NCBI GeneID: 100133941
• Molecular Weight: 8,097 Da
• NCBI Official Full Name: CD24
• NCBI Official Synonym Full Names: CD24 molecule
• NCBI Official Symbol: CD24
• NCBI Official Synonym Symbols: CD24A
• NCBI Protein Information: signal transducer CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• UniProt Protein Name: Signal transducer CD24
• Protein Family: Signal transducer
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A
• UniProt Entry Name: CD24_HUMAN

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function: signal transducer activity; protein binding; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; elevation of cytosolic calcium ion concentration; cholesterol homeostasis; cell-cell adhesion; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; T cell costimulation; regulation of epithelial cell differentiation; negative regulation of transforming growth factor-beta3 production; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To