Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Kininogen-1 antibody at 1:200 dilution.)
Rabbit Kininogen 1 Polyclonal Antibody
Affinity purified Rabbit polyclonal Kininogen 1 antibody
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Kininogen 1 antibody was purified by antigen-affinity chromatography
Synonyms
Kininogen 1; Polyclonal Antibody; Affinity purified Rabbit polyclonal Kininogen 1 antibody; Polyclonal Kininogen 1; Anti-Kininogen 1;; high molecular weight kininogen; anti-Kininogen 1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Kininogen 1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Kininogen 1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)-Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000
Immunogen
Kininogen 1 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 416 of Kininogen 1
Cross-Reactivity
Human
Preparation and Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Kininogen-1 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Kininogen-1 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 10 % SDS PAGE gel and Kininogen 1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 10 % SDS PAGE gel and Kininogen 1 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using Kininogen 1 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using Kininogen 1 antibody at a dilution of 1:500)
Related Product Information for anti-Kininogen 1 antibody
High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein -kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy.
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Product Notes
The Kininogen 1 (Catalog #AAA5309026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Kininogen 1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)-Paraffin, Western Blot (WB). IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000. Researchers should empirically determine the suitability of the Kininogen 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kininogen 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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