ZAP70 recombinant protein

ZAP70 protein

Cat. Num. AAA5303958

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Purity: ZAP70 protein was purified using a a proprietary chromatographic technique.
• Synonyms: ZAP70; ZAP70 protein; ZAP-70; zeta-chain (TCR) associated protein kinase 70kDa protein; ZAP-70 protein; ZAP 70 protein; ZAP 70; ZAP70 recombinant protein

• Host: Human
• Purity/Purification: ZAP70 protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in a storage buffer consisting of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol
• Sequence Length: 493

• Protein Type: Recombinant
• Biological Significance: ZAP70 is a non-receptor protein tyrosine kinase that is involved in signaling by the T-cell antigen receptor. Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70. TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The phosphorylation of ZAP70 after MHC-I ligation is dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex.
• Expression System: Sf-9 insect cells
• Preparation and Storage: Store at -80 degree C

Related Product Information for ZAP70 recombinant protein

Purified recombinant Human ZAP70 protein

Product Categories/Family for ZAP70 recombinant protein

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 24657846
• NCBI GeneID: 7535
• Molecular Weight: 96 kDa
• NCBI Official Full Name: ZAP70 protein
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; T cell activation; B cell activation; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; protein amino acid phosphorylation; positive regulation of T cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; beta selection; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; T cell differentiation; positive thymic T cell selection

Disease: Selective T-cell Defect