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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human Histone H1oo Polyclonal Antibody | anti-H1FOO antibody

Histone H1oo Antibody

Gene Names
H1FOO; H1.8; H1oo; osH1
Reactivity
Human
Applications
Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
Histone H1oo; Polyclonal Antibody; Histone H1oo Antibody; H1OO; OSH1; Oocyte-specific histone H1; Oocyte-specific linker histone H1; osH1; anti-H1FOO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Histone H1oo Antibody detects endogenous levels of total Histone H1oo protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
207
Applicable Applications for anti-H1FOO antibody
Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:100~1:500
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1.
Immunogen Range
131-180
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using Histone H1 Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21 kDa
NCBI Official Full Name
histone H1oo isoform 2
NCBI Official Synonym Full Names
H1 histone family member O oocyte specific
NCBI Official Symbol
H1FOO
NCBI Official Synonym Symbols
H1.8; H1oo; osH1
NCBI Protein Information
histone H1oo
UniProt Protein Name
Histone H1oo
Protein Family
UniProt Gene Name
H1FOO
UniProt Synonym Gene Names
H1OO; OSH1; osH1

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015]

Uniprot Description

May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling ().

Research Articles on H1FOO

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Product Notes

The H1FOO h1foo (Catalog #AAA9507801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H1oo Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1oo can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA). IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the H1FOO h1foo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1oo, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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