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Testing Data

Mouse NK Cells (NK1.1) Monoclonal Antibody

Anti-Mouse NK Cells (NK1.1), PE (Clone PK136) (mouse IgG2a)

Applications
Immunohistochemistry
Synonyms
NK Cells (NK1.1); Monoclonal Antibody; Anti-Mouse NK Cells (NK1.1); PE (Clone PK136) (mouse IgG2a); PE Anti-Mouse NK Cells Monoclonal Antibody; anti-NK Cells (NK1.1) antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
PK136
Specificity
Mouse NK Cells (NK1.1)
Form/Format
Biotin/FITC/APC/AF488/AF647/AF700 conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Applicable Applications for anti-NK Cells (NK1.1) antibody
Immunohistochemistry (IHC)
Application Notes
This antibody is suitable for use in flow cytometry.
Immunogen
NK-1 + cells from mouse spleen and bone marrow.
Preparation and Storage
For all formats, store at 4°C. DO NOT FREEZE APC, PE, AF488, AF647 and AF700 conjugates. For long term storage (Purified, Biotin and FITC), aliquot and freeze unused portion at -20?C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Testing Data

Testing Data

Immunohistochemistry

(Purified Anti-Mouse NK Cells (NK1.1) (MBS520387) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 2.5x (Left) and 40x (Right) magnification. (Anti-mouse NK Cells (NK1.1) (MBS520387) was used at a dilution of 1:100 and stained with DAB for 1 minute))

Immunohistochemistry (Purified Anti-Mouse NK Cells (NK1.1) (MBS520387) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 2.5x (Left) and 40x (Right) magnification. (Anti-mouse NK Cells (NK1.1) (MBS520387) was used at a dilution of 1:100 and stained with DAB for 1 minute))
Related Product Information for anti-NK Cells (NK1.1) antibody
Anti-mouse NK cells monoclonal antibody is specific for recognizing mouse NK cells in selected strains of mice (i.e.C57BL, FVB/N, NZB but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, SJL and 129). Clone PK136 is specific for mouse NK1.1, also known as NKR-PIC and Ly 55. Mouse NK1.1 is expressed on NK cells and NKT cells on the following strains: C57BL, FVB/N and NZB. There are published reports that PK136 mAb binds to NKRPIB on SJL/K NK cells4.

This antibody is suitable for use in flow cytometry and with paraffin-embedded Sections. This clone has been reported to work for in-vitro and in-vivo depletion assays.

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Product Notes

The NK Cells (NK1.1) (Catalog #AAA520386) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NK Cells (NK1.1) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). This antibody is suitable for use in flow cytometry. Researchers should empirically determine the suitability of the NK Cells (NK1.1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NK Cells (NK1.1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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