TRIO recombinant protein

TRIO, Active

Cat. Num. AAA517694

• Gene Names: TRIO; tgat; ARHGEF23
• Applications: Western Blot
• Synonyms: TRIO; Active; TRIO recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for TRIO recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for TRIO recombinant protein

Recombinant human TRIO (2627-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The TRIO protein accession number is O75962-1.
TRIO (Triple functional domain protein) belongs to CAMK Ser/Thr protein kinase family with 5 isoforms produced by alternative splicing. It encodes two Dbl-homology-Pleckstrin-homology (DH-PH) Rho-GEF units with different specificities and a C-terminal serine/threonine kinase domain. It is a Rho-guanine nucleotide exchange factors (GEFs) that catalyzes exchange of GDP for GTP on small Rho-GTPases, such as Rac1, RhoG and RhoA. TRIO was originally identified as a binding partner of the transmembrane tyrosine phosphatase LAR, and they together play a role in coordinating cell-matrix and cytoskeletal rearrangements required for cell migration and cell growth.

Product Categories/Family for TRIO recombinant protein

Protein Kinases; Cancer; ERK/MAPK Pathway; Neurobiology; Ser/Thr Kinases

References

1. Debant A, et al. The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains. Proc Natl Acad Sci U S A. 93:5466-71. 1996.
2. van Rijssel J, et al: The many faces of the guanine-nucleotide exchange factor trio. Cell Adh Migr. 6:482-7. 2012.

NCBI and Uniprot Product Information

• NCBI GI #: 45439359
• NCBI GeneID: 7204
• NCBI Accession #: NP_009049.2
• NCBI GenBank Nucleotide #: NM_007118.3
• UniProt Accession #: O75962; Q13458; Q59EQ7; Q6PJC9; Q6ZN05; Q8IWK8; D3DTD1
• Molecular Weight: 287,377 Da
• NCBI Official Full Name: triple functional domain protein
• NCBI Official Synonym Full Names: trio Rho guanine nucleotide exchange factor
• NCBI Official Symbol: TRIO
• NCBI Official Synonym Symbols: tgat; ARHGEF23
• NCBI Protein Information: triple functional domain protein
• UniProt Protein Name: Triple functional domain protein
• Protein Family: Triostin synthetase
• UniProt Gene Name: TRIO
• UniProt Entry Name: TRIO_HUMAN

NCBI Description

This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

TRIO: Promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Apoptosis; EC 2.7.11.1; GEFs, misc.; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; GEFs; CAMK group; Trio family

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytosol

Molecular Function: ATP binding; enzyme binding; guanyl-nucleotide exchange factor activity; protein binding; protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of apoptosis; positive regulation of GTPase activity; protein amino acid phosphorylation; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; transmembrane receptor protein tyrosine phosphatase signaling pathway