Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
NEK4 recombinant protein
NEK4, Active
Gene Names
NEK4; NRK2; STK2; pp12301
Applications
Western Blot
Synonyms
NEK4; Active; NRK2; STK2; NEK4 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for NEK4 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for NEK4 recombinant protein
Recombinant full-length human NEK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC063044.
NEK4 or Never In Mitosis A-related kinase 4 is a serine/threonine protein kinase. Northern blot show NEK4 is expressed as 2 mRNAs of 4.0 kb and 6.8 kb in heart and several other tissues. NEK4 gene encodes an 841-amino acid polypeptide and the levels of NEK4 mRNA remain invariant throughout the cell cycle (1). Nek4 has been identified in a genetic screen as a mediator of response to Taxol (2). After Taxol treatment, NEK4 promoted microtubule outgrowth, whereas NEK4 deficiency impaired G(2)-M arrest and decreased formation of mitotic-like asters. In contrast, NEK4 deficiency sensitized cells to vincristine, which destabilizes microtubules.
NEK4 or Never In Mitosis A-related kinase 4 is a serine/threonine protein kinase. Northern blot show NEK4 is expressed as 2 mRNAs of 4.0 kb and 6.8 kb in heart and several other tissues. NEK4 gene encodes an 841-amino acid polypeptide and the levels of NEK4 mRNA remain invariant throughout the cell cycle (1). Nek4 has been identified in a genetic screen as a mediator of response to Taxol (2). After Taxol treatment, NEK4 promoted microtubule outgrowth, whereas NEK4 deficiency impaired G(2)-M arrest and decreased formation of mitotic-like asters. In contrast, NEK4 deficiency sensitized cells to vincristine, which destabilizes microtubules.
Product Categories/Family for NEK4 recombinant protein
References
1. Levedakou, E. N., et.al: Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988, 1994.
2. Doles, J. et al: Nek4 status differentially alters sensitivity to distinct microtubule poisons. Cancer Res. 2010 Feb 1;70(3):1033-41.
2. Doles, J. et al: Nek4 status differentially alters sensitivity to distinct microtubule poisons. Cancer Res. 2010 Feb 1;70(3):1033-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
160.0 kDa
NCBI Official Full Name
Homo sapiens NIMA (never in mitosis gene a)-related kinase 4, mRNA
NCBI Official Synonym Full Names
NIMA related kinase 4
NCBI Official Symbol
NEK4
NCBI Official Synonym Symbols
NRK2; STK2; pp12301
NCBI Protein Information
serine/threonine-protein kinase Nek4
Protein Family
NCBI Description
The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017]
Research Articles on NEK4
Similar Products
Product Notes
The NEK4 (Catalog #AAA517306) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NEK4 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the NEK4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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