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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MEK6 (MAP2K6) Recombinant Protein | MEK6 recombinant protein
MEK6 (MAP2K6), Unactive
Applications
Western Blot
Synonyms
MEK6 (MAP2K6); Unactive; MAP2K6; MEK6; MAPKK6; PRKMK6; SAPKK3; SKK3; MEK6 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for MEK6 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for MEK6 recombinant protein
Recombinant full-length human MEK6 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. The gene accession number is NM_002758.
Mitogen-acitvated protein kinase kinase 6 (MEK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1). MEK6 is a dual-specificity protein kinase that activates p38 MAPK. MEK6 phosphorylates p38 MAP kinase at its activation site Thr-Gly-Tyr and this stimulates the ability of p38 MAPK to phosphorylate protein substrates such as ATF-2 and Elk-1. MEK6 is activated by different forms of cellular stress and inflammatory cytokines (2). Activation of MEK6 occurs via phosphorylation of Ser207 and Thr211 on MEK6 by upstream protein kinases.
Mitogen-acitvated protein kinase kinase 6 (MEK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1). MEK6 is a dual-specificity protein kinase that activates p38 MAPK. MEK6 phosphorylates p38 MAP kinase at its activation site Thr-Gly-Tyr and this stimulates the ability of p38 MAPK to phosphorylate protein substrates such as ATF-2 and Elk-1. MEK6 is activated by different forms of cellular stress and inflammatory cytokines (2). Activation of MEK6 occurs via phosphorylation of Ser207 and Thr211 on MEK6 by upstream protein kinases.
Product Categories/Family for MEK6 recombinant protein
References
1. Dérijard, B. et al: Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms. Science. 1995; 269(5220):17.
2. Han, J. et al. Characterization of the structure and function of a novel MAP kinase kinase (MEK6). J. Biol. Chem. 1996;271(6):2886-91.
2. Han, J. et al. Characterization of the structure and function of a novel MAP kinase kinase (MEK6). J. Biol. Chem. 1996;271(6):2886-91.
NCBI and Uniprot Product Information
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Product Notes
The MEK6 (Catalog #AAA517183) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MEK6 (MAP2K6) can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the MEK6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK6 (MAP2K6), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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