Rabbit anti-Human TSPY Polyclonal Antibody | anti-TSPY antibody

TSPY, CT (TSPY1, TSPY, Testis-specific Y-encoded protein 1, Cancer/testis antigen 78) (Azide free) (HRP)

Cat. Num. AAA6359654

• Gene Names: TSPY3; CT78
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSPY; Polyclonal Antibody; CT (TSPY1; Testis-specific Y-encoded protein 1; Cancer/testis antigen 78) (Azide free) (HRP); TSPY1; Cancer/testis antigen 78; anti-TSPY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TSPY antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TSPY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-309 amino acids from the C-terminal region of human TSPY.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TSPY Antibody (C-term) pre-incubated without(lane 1) and with(lane 2) blocking peptide in LNCAP cell line lysate. TSPY Antibody (C-term) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TSPY Antibody (C-term) (AP14884b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSPY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TSPY antibody

TSPY may be involved in sperm differentiation and proliferation.

Product Categories/Family for anti-TSPY antibody

Antibodies; Cancer Markers

References

Kimura, K., et al. Genome Res. 16(1):55-65(2006). Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 303228330
• NCBI GeneID: 728137
• NCBI Accession #: NP_001071165.2; XP_002344239.1
• NCBI GenBank Nucleotide #: NM_001077697.2
• UniProt Accession #: Q01534; P0CV98
• Molecular Weight: 35,101 Da
• NCBI Official Full Name: testis-specific Y-encoded protein 3
• NCBI Official Synonym Full Names: testis specific protein, Y-linked 3
• NCBI Official Symbol: TSPY3
• NCBI Official Synonym Symbols: CT78
• NCBI Protein Information: testis-specific Y-encoded protein 3
• UniProt Protein Name: Testis-specific Y-encoded protein 1
• UniProt Gene Name: TSPY1
• UniProt Synonym Gene Names: TSPY; CT78
• UniProt Entry Name: TSPY1_HUMAN

Uniprot Description

TSPY1: May be involved in sperm differentiation and proliferation. TSPY is located in the gonadoblastoma critical region and is preferentially expressed in tumor germ cells of gonadoblastoma specimens. Expression also correlates with testicular seminoma and tumorigenesis of the prostate gland. Belongs to the nucleosome assembly protein (NAP) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Yp11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: cell proliferation; nucleosome assembly; gonadal mesoderm development; spermatogenesis; cell differentiation; sex differentiation

Disease: Gonadoblastoma