SDS-Page
(Purity: Recombinant Human IL6 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)
IL6 recombinant protein
IL6
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Applications
Cell Assay
Synonyms
IL6; IL-6; B cell differentiation factor; BCDF; B cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; HPGF; HSF; MGI-2; Hybridoma growth factor; Interferon beta-2; IFN-beta 2; Recombinant Human Interleukin-6 (IL6); IL6 recombinant protein
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered solution in PBS (pH7.4)
Applicable Applications for IL6 recombinant protein
Cell Assay
Species
Human
Endotoxin Level
<1.0EU/ug of recombinant protein as determined by the LAL method
Sample Cell Assay Data
The ED50 as determined by the dose-dependent stimulation of human TF-1 cells was in the range of 0.5-1ng/mL
Reconstitution
A quick spin of the vial followed by reconstitution in sterile PBS to a concentration not less than 0.1mg/ml.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
SDS-Page
(Purity: Recombinant Human IL6 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)
SDS-Page
(Purity: Recombinant Human IL6 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)
Related Product Information for IL6 recombinant protein
Recombinant Human IL6 was expressed in E Coli cells. The protein accession number is P05231
IL6 is a pleiotropic cytokine that participates in a wide variety of biological functions such as acute phase response, inflammation, hematopoiesis, bone metabolism and cancer. While produced mainly by stimulated monocytes, fibroblasts and endothelial cells, IL6 is also known to be produced by macrophages, T cells, B lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells and keratinocytes, upon stimulation. Unlike murine IL6 which is inactive on human cells, both human and murine IL6 are equally active on murine cells. The IL6 receptor is a strongly glycosylated protein of 80kDa and a length of 449 amino acids (designated CD126). Recombinant human IL6 is a 21kDa, non-glycosylated protein that contains two disulfide bridges.
IL6 is a pleiotropic cytokine that participates in a wide variety of biological functions such as acute phase response, inflammation, hematopoiesis, bone metabolism and cancer. While produced mainly by stimulated monocytes, fibroblasts and endothelial cells, IL6 is also known to be produced by macrophages, T cells, B lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells and keratinocytes, upon stimulation. Unlike murine IL6 which is inactive on human cells, both human and murine IL6 are equally active on murine cells. The IL6 receptor is a strongly glycosylated protein of 80kDa and a length of 449 amino acids (designated CD126). Recombinant human IL6 is a 21kDa, non-glycosylated protein that contains two disulfide bridges.
Product Categories/Family for IL6 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
212
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN
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Product Notes
The IL6 il6 (Catalog #AAA517108) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL6 can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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