Human IL-6 ELISA Kit | IL6 elisa kit

Human IL-6 Quick ELISA Kit

Cat. Num. AAA1753911

• Gene Names: IL6; HGF; HSF; BSF2; IL-6; IFNB2
• Reactivity: Human
• Synonyms: IL-6; Human IL-6 Quick ELISA Kit; B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon; beta 2); interleukin BSF-2; interleukin-6; MGI-2A; interleukin 6; IL6 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL6

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 4.69pg/ml-300pg/ml
• Sensitivity: <0.3pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|4.69|9.38|18.75|37.5|75|150|300; O.D.|0.006|0.081|0.145|0.276|0.486|0.952|1.613|2.474
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|15|42|134|; Standard deviation|0.88|2.26|10.31|; CV(%)|5.9%|5.4%|7.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|13|46|130; Standard deviation|1.00|2.57|11.05; CV(%)|7.5%|5.6%|8.5%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 15|11|10|13|12|0.99|8.3%; Sample 2: 42|45|38|40|41|1.96|5.1%; Sample 3: 134|145|151|147|144|3.6|2.5%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: P29-M212
• Anticoagulant: heparin, EDTA or citrate
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human IL-6 with <0.3pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human IL6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL6 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL6. The detection antibody is a HRP linked goat polyclonal antibody. The kit contains recombinant Human IL6 with immunogen: Expression system for standard: E Coli; Immunogen sequence: P29-M212. The kit is analytically validated with ready to use reagents.; ; To measure Human IL6, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL6 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL6 in the sample.
• Assay Procedure Step 1: 300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.75pg/ml, 9.375pg/ml, 4.6875pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for IL6 elisa kit

The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20. 3kDa.

Product Categories/Family for IL6 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 124347
• NCBI GeneID: 3569
• UniProt Accession #: P05231; Q9UCU2; Q9UCU3; Q9UCU4
• Molecular Weight: 212
• NCBI Official Full Name: Interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: HGF; HSF; BSF2; IL-6; IFNB2
• NCBI Protein Information: interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
• UniProt Entry Name: IL6_HUMAN