Western Blot (WB)
(Sample Data: Western blot analysis using Anti-phospho-Cyclin B1 antibody (1:100) to detect phosphorylated human Cyclin B1 (~48kDa). lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 ug of lysates)
Rabbit Cyclin B1 Polyclonal Antibody
Anti-Phospho-Cyclin B1 (Ser126)
Reactivity
Human, Rat, Dog, Chimpanzee, Mouse, Hamster
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Immunoaffinity chromatography followed by solid phase adsorption
Synonyms
Cyclin B1; Polyclonal Antibody; Anti-Phospho-Cyclin B1 (Ser126); anti-Cyclin B1 antibody
Host
Rabbit
Reactivity
Human, Rat, Dog, Chimpanzee, Mouse, Hamster
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human Cylcin B1 protein phosphorylated at Serine126
Purity/Purification
Immunoaffinity chromatography followed by solid phase adsorption
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Cyclin B1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 12-131 of Human Cyclin B1 protein
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis using Anti-phospho-Cyclin B1 antibody (1:100) to detect phosphorylated human Cyclin B1 (~48kDa). lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 ug of lysates)
Western Blot (WB)
(Sample Data: Western blot analysis using Anti-phospho-Cyclin B1 antibody (1:100) to detect phosphorylated human Cyclin B1 (~48kDa). lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 ug of lysates)
Related Product Information for anti-Cyclin B1 antibody
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Product Categories/Family for anti-Cyclin B1 antibody
References
1. Giordano, A., et al.: A 60 kd cdc-2 associated polypeptide complexes with the E1A proteins of adenovirus-infected cells. Cell. 1989: 58; 981-990.
2. Pines, J., et al.: Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990: 346; 760-763.
2. Pines, J., et al.: Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990: 346; 760-763.
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Product Notes
The Cyclin B1 (Catalog #AAA515922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Cyclin B1 (Ser126) reacts with Human, Rat, Dog, Chimpanzee, Mouse, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Cyclin B1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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