Western Blot (WB)
(Sample Data: Western Blot of Anti-Phospho-ATM (Ser1981) antibody (1:1,000). Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 ug per lane.)
Sheep anti-Human ATM Polyclonal Antibody | anti-Phospho-ATM antibody
Anti-Phospho-ATM (Ser1981)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity chromatography
Synonyms
ATM; Polyclonal Antibody; Anti-Phospho-ATM (Ser1981); anti-Phospho-ATM antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
Sheep, IgG
Specificity
Recognizes the ATM protein phosphorylated at Serine 1981
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospho-ATM antibody
ELISA (EIA), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western Blot of Anti-Phospho-ATM (Ser1981) antibody (1:1,000). Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 ug per lane.)
Western Blot (WB)
(Sample Data: Western Blot of Anti-Phospho-ATM (Ser1981) antibody (1:1,000). Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 ug per lane.)
Related Product Information for anti-Phospho-ATM antibody
ATM is a master regulator for dsDNA break repair, which belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily. Unlike other lipid kinases, ATM phosphorylates proteins. ATM is present in undamaged cells as an inactive dimer, which is activated likely in response to changes in chromatin structure. Exposure of cells to ionizing radiation triggers ATM kinase activity, leading to cell cycle arrest in the G1, S or G2 phase. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, MDM2 and CHK2 in the G1 checkpoint; NBS1, BRCA1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and BRCA1 and hRAD17 during the G2/M checkpoint. ATM is mutated in the hereditary disease ataxia-telangiectasia.
Product Categories/Family for anti-Phospho-ATM antibody
References
1. Bakkenist, C. J., et al.: DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature. 2003: 421; 499-506.
2. Kitagawa R, et al.: Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004: 18(12);1423-38.
3. Falck, J. et al.: Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005: 434; 605-611.
2. Kitagawa R, et al.: Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004: 18(12);1423-38.
3. Falck, J. et al.: Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005: 434; 605-611.
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Product Notes
The Phospho-ATM (Catalog #AAA515830) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-ATM (Ser1981) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the Phospho-ATM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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