CDK3, recombinant protein

CDK3, recombinant protein

Cat. Num. AAA515399

• Applications: Western Blot
• Synonyms: CDK3; ; recombinant protein; MAP2K1; MKK1; MAPKK1; PRKMK1; Unactive

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1584

• Applicable Applications for CDK3, recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: N-terminal GST
• Source Note: Recombinant human full-length CDK3 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Product Categories/Family for CDK3, recombinant protein

Unactive Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 164519034
• NCBI GeneID: 1018
• NCBI Accession #: NP_001249.1
• NCBI GenBank Nucleotide #: NM_001258
• UniProt Accession #: Q00526
• Molecular Weight: 60 kDa
• NCBI Official Full Name: Homo sapiens cyclin-dependent kinase 3 (CDK3), mRNA
• NCBI Official Synonym Full Names: cyclin-dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3; cell division protein kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3
• UniProt Entry Name: CDK3_HUMAN

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner. Ref.3 Ref.4 Ref.5

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with CABLES1 and CABLES2

By similarity. Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation. Ref.4 Ref.5

Tissue specificity: Expressed in cancer cell lines and glioblastoma tissue. Ref.5

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.